PUBCHEM_BIOASSAY: qHTS Inhibitors of AmpC Beta-Lactamase (assay with detergent). (Class of assay: confirmatory) [Related pubchem assays: 1002 (Confirmation Concentration-Response Assay for Inhibitors of AmpC Beta-Lactamase (assay with detergent)), 585 (Promiscuous and Specific Inhibitors of AmpC Beta-Lactamase (assay without detergent) - a screen old NIH MLSMR collection), 584 (Promiscuous and Specific Inhibitors of AmpC Beta-Lactamase (assay with detergent) - a screen of the old NIH MLSMR collection), 1003 (Confirmation Cuvette-Based Assay for Inhibitors of AmpC Beta-Lactamase (assay with detergent))]
PUBCHEM_BIOASSAY: qHTS Assay for Inhibitors of the Phosphatase Activity of Eya2. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID488939]
PUBCHEM_BIOASSAY: Inhibitors of Eya2ED: Hit Confirmation in Primary Screen. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID488837, AID488939]
PubChem BioAssay. A quantitative high throughput screen for small molecules that induce DNA re-replication in MCF 10a normal breast cells. (Class of assay: confirmatory)
Inhibition of GST-fused human Eya2 ED (253 to 538 residues) expressed in Escherichia coli pre-incubated for 10 mins followed by OMFP substrate addition and further incubated for 30 mins by fluorescence based assay
Inhibition of Scp1 (unknown origin) up to 100 uM pre-incubated for 10 mins followed by OMFP substrate addition and further incubated for 30 mins by fluorescence based assay
Inhibition of GST-fused human Eya2 ED (253 to 538 residues) expressed in Escherichia coli pre-incubated for 10 mins before phosphorylated H2AX peptide substrate addition and further incubated for 40 mins by malachite green-based secondary phosphatase assay
Inhibition of human recombinant PPM1A expressed in Escherichia coli up to 100 uM pre-incubated for 10 mins followed by OMFP substrate addition and further incubated for 30 mins by fluorescence based assay
Inhibition of N-terminal Met and 6-His tagged human recombinant PTP1B (Glu2 to Asn321 residues) expressed in Escherichia coli up to 100 uM pre-incubated for 10 mins followed by OMFP substrate addition and further incubated for 30 mins by fluorescence based assay
Binding affinity to GST-fused human Eya2 ED (253 to 538 residues) expressed in Escherichia coli assessed as induction of protein destabilization by measuring reduction in melting temperature in presence of 5 uM Mg2+ ions by thermal shift assay
Binding affinity to GST-fused human Eya2 ED (253 to 538 residues) expressed in Escherichia coli assessed as induction of protein stabilization by measuring melting temperature in presence of EDTA by thermal shift assay