| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3227714 | B | Inhibition of mouse PI3K alpha expressed in insect cells coexpressing p85 subunit assessed as PI(3,4,5)P3 formation using PI(4,5)P2 as substrate by measuring displacement of TAMRA-labeled PI(3,4,5)P3 from Grip1 PH domain after 35 mins by ligand displacement fluorescence polarization assay | Mus musculus | 9 | ALA3217449 | cell-based format | Scientific Literature | |
| 2. | ALA3227715 | B | Inhibition of recombinant mTOR (unknown origin) assessed as GFP-4E-BP1 substrate phosphorylation after 20 mins by TR-FRET assay | Homo sapiens | 9 | ALA3217449 | single protein format | Scientific Literature | |
| 3. | ALA3225914 | B | Inhibition of AKT phosphorylation at S473 in human BT20 cells after 1 hr by sandwich ELISA | Homo sapiens | 5 | ALA3217449 | cell-based format | Scientific Literature | |
| 4. | ALA3225915 | A | Metabolic stability in human liver microsomes assessed as extraction ratio | Homo sapiens | 7 | ALA3217449 | assay format | Scientific Literature | |
| 5. | ALA3225916 | P | Kinetic solubility of the compound | 7 | ALA3217449 | small-molecule physicochemical format | Scientific Literature | ||
| 6. | ALA3225925 | A | Drug metabolism in iv dosed rat plasma assessed as 2-amino-8-((trans)-4-hydroxycyclohexyl)-6-(6-methoxypyridin-3-yl)-4-methylpyrido[2,3-d]pyrimidin-7(8H)-one level | Rattus norvegicus | 1 | ALA3217449 | organism-based format | Scientific Literature | |
| 7. | ALA3225927 | A | Drug metabolism in po dosed mouse plasma assessed as 2-amino-8-((trans)-4-hydroxycyclohexyl)-6-(6-methoxypyridin-3-yl)-4-methylpyrido[2,3-d]pyrimidin-7(8H)-one level | Mus musculus | 1 | ALA3217449 | organism-based format | Scientific Literature | |
| 8. | ALA3706092 | B | Biochemical Assay: Compounds of the present invention were evaluated for potency against PI3-Kα using an in vitro kinase assay. PI3-Kα activity is measured in vitro by determining the level of phosphorylation of the substrate PI(4,5)P2. The formation of product PI(3,4,5)P3 is monitored by binding to the Grip1 PH domain in a ligand displacement fluorescence polarization (FP) assay, in which the TAMRA-labeled PI(3,4,5)P3 complexed with Grip1 PH domain is displaced by PI(3,4,5)P3 formed in the PI3-Kα reaction resulting in a decrease in FP signal. | Homo sapiens | 173 | ALA3639030 | single protein format | BindingDB Database |
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