# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3225545 | F | Antagonist activity at human recombinant histamine H2 receptor-Gsalphas fusion protein expressed in Sf9 cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding after 90 mins by liquid scintillation counting | Homo sapiens | 21 | ALA3217456 | cell-based format | Scientific Literature | |
2. | ALA3225546 | F | Intrinsic activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding at 10 uM after 90 mins by liquid scintillation counting relative to histamine | Homo sapiens | 16 | ALA3217456 | cell-based format | Scientific Literature | |
3. | ALA3225547 | F | Intrinsic activity at human recombinant histamine H3 receptor expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 and RGS4 assessed as [35S]GTPgammaS binding at 10 uM after 90 mins by liquid scintillation counting relative to histamine | Homo sapiens | 18 | ALA3217456 | cell-based format | Scientific Literature | |
4. | ALA3225548 | F | Intrinsic activity at human recombinant histamine H2 receptor-Gsalphas fusion protein expressed in Sf9 cells assessed as [35S]GTPgammaS binding at 10 uM after 90 mins by liquid scintillation counting relative to histamine | Homo sapiens | 21 | ALA3217456 | cell-based format | Scientific Literature | |
5. | ALA3225549 | F | Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting | Homo sapiens | 19 | ALA3217456 | cell-based format | Scientific Literature | |
6. | ALA3225551 | F | Agonist activity at human recombinant histamine H3 receptor expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 and RGS4 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting | Homo sapiens | 13 | ALA3217456 | cell-based format | Scientific Literature | |
7. | ALA3225555 | F | Antagonist activity at human recombinant histamine H1 receptor expressed in Sf9 cells coexpressing RGS4 assessed as inhibition of histamine-induced [gamma33P]GTP hydrolysis preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay | Homo sapiens | 35 | ALA3217456 | cell-based format | Scientific Literature | |
8. | ALA3225906 | B | Intrinsic activity at human recombinant histamine H1 receptor expressed in Sf9 cells coexpressing RGS4 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay relative to histamine | Homo sapiens | 32 | ALA3217456 | cell-based format | Scientific Literature | |
9. | ALA3253587 | B | Antagonist at histamine H2 receptor in guinea pig atrium assessed as inhibition of histamine-induced response | Cavia porcellus | 12 | ALA3244335 | single protein format | Scientific Literature | |
10. | ALA3253588 | B | Antagonist at histamine H2 receptor in rat uterus assessed as inhibition of histamine-induced response | Rattus norvegicus | 12 | ALA3244335 | tissue-based format | Scientific Literature |