# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3239920 | B | Inhibition of SARS coronavirus papain-like protease (amino acids 1541 to 1855) expressed in Escherichia coli BL21 (DE3) cells assessed as decrease in AMC release using Z-RLRGG-AMC as substrate by multimode plate reader analysis | SARS coronavirus | 31 | ALA3232931 | cell-based format | Scientific Literature | |
2. | ALA3239921 | B | Inhibition of SARS coronavirus papain-like protease Q270A mutant (amino acids 1541 to 1855) expressed in Escherichia coli BL21 (DE3) cells assessed as decrease in AMC release using Z-RLRGG-AMC as substrate by multimode plate reader analysis | SARS coronavirus | 4 | ALA3232931 | cell-based format | Scientific Literature | |
3. | ALA3239922 | B | Inhibition of SARS coronavirus papain-like protease Q270E mutant (amino acids 1541 to 1855) expressed in Escherichia coli BL21 (DE3) cells assessed as decrease in AMC release using Z-RLRGG-AMC as substrate by multimode plate reader analysis | SARS coronavirus | 4 | ALA3232931 | cell-based format | Scientific Literature | |
4. | ALA3239923 | B | Inhibition of SARS coronavirus papain-like protease Q270D mutant (amino acids 1541 to 1855) expressed in Escherichia coli BL21 (DE3) cells assessed as decrease in AMC release using Z-RLRGG-AMC as substrate by multimode plate reader analysis | SARS coronavirus | 4 | ALA3232931 | cell-based format | Scientific Literature | |
5. | ALA3239942 | B | Inhibition of human coronavirus NL63 PLP2 (amino acids 1565 to 1894) expressed in Escherichia coli BL21 (DE3) cells assessed as reduction of AMC release using Z-RLRGG-AMC as substrate by multimode plate reader analysis | Human coronavirus NL63 | 6 | ALA3232931 | cell-based format | Scientific Literature | |
6. | ALA3238618 | B | Inhibition of human coronavirus NL63 PLP2 (amino acids 1565 to 1894) expressed in Escherichia coli BL21 (DE3) cells assessed as reduction of AMC release using Z-RLRGG-AMC as substrate at 100 uM by multimode plate reader analysis | Human coronavirus NL63 | 6 | ALA3232931 | cell-based format | Scientific Literature | |
7. | ALA3238619 | F | Antiviral activity against SARS coronavirus infected in african green monkey Vero E6 cells assessed as cell viability after 48 hrs by Cell Titer-Glo luminescent assay | Severe acute respiratory syndrome-related coronavirus | 9 | ALA3232931 | organism-based format | Scientific Literature | |
8. | ALA3238620 | A | Cytotoxicity against african green monkey Vero E6 cells assessed as cell viability after 48 hrs by Cell Titer-Glo luminescent assay | Chlorocebus sabaeus | 6 | ALA3232931 | cell-based format | Scientific Literature | |
9. | ALA3238621 | A | Therapeutic index, ratio of CC50 for african green monkey Vero E6 cells to EC50 for SARS coronavirus infected in african green monkey Vero E6 cells | 6 | ALA3232931 | cell-based format | Scientific Literature | ||
10. | ALA3238622 | A | Cytotoxicity against human HEK293 cells assessed as cell viability after 48 hrs by Cell Titer-Glo luminescent assay | Homo sapiens | 6 | ALA3232931 | cell-based format | Scientific Literature | |
11. | ALA3238623 | A | Metabolic stability in mouse liver microsomes assessed as parent compound remaining at 1 uM after 15 mins | Mus musculus | 4 | ALA3232931 | assay format | Scientific Literature | |
12. | ALA3238624 | A | Half life in mouse liver microsomes at 1 uM | Mus musculus | 4 | ALA3232931 | assay format | Scientific Literature | |
13. | ALA3238625 | B | Inhibition of SARS coronavirus papain-like protease (amino acids 1541 to 1855) expressed in Escherichia coli BL21 (DE3) cells assessed as decrease in AMC release using Z-RLRGG-AMC as substrate by serum shift assay based multimode plate reader analysis in presence of 5 to 20% human serum albumin | SARS coronavirus | 6 | ALA3232931 | cell-based format | Scientific Literature | |
14. | ALA5109239 | B | Inhibition of full-length SARS-CoV-2 PLpro expressed in Escherichia coli BL21(DE3) using HCC-RLRGG-NH(CH2)4NH-DABCYL probe as substrate incubated for 30 mins by enzymatic assay | Severe acute respiratory syndrome coronavirus 2 | 10 | ALA5107976 | assay format | Scientific Literature | |
15. | ALA5109240 | B | Inhibition of full-length SARS-CoV-2 PLpro expressed in Escherichia coli BL21(DE3) using Ub-Rho as substrate incubated for 5 mins by enzymatic assay | Severe acute respiratory syndrome coronavirus 2 | 10 | ALA5107976 | assay format | Scientific Literature |