# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3239746 | B | Activation of Nrf2 in rat PC12 cells assessed as increase of HO-1 mRNA level at 1 to 4 uM by qRT-PCR analysis | Rattus norvegicus | 1 | ALA3232949 | cell-based format | Scientific Literature | |
2. | ALA3239747 | B | Inhibition of Keap1-Nrf2 (unknown origin) interaction assessed as compound's equilibrium dissociation constant after 60 mins by fluorescence anisotropy assay | Homo sapiens | 54 | ALA3232949 | protein format | Scientific Literature | |
3. | ALA3239748 | B | Binding affinity to kelch domain of Keap1 (unknown origin) assessed as change in melting temperature after 15 mins by differential scanning fluorimetry assay | Homo sapiens | 7 | ALA3232949 | single protein format | Scientific Literature | |
4. | ALA3239780 | B | Activation of Nrf2 in rat PC12 cells assessed as increase of HO-1 mRNA level after 4.5 to 9 hrs by qRT-PCR analysis | Rattus norvegicus | 1 | ALA3232949 | cell-based format | Scientific Literature | |
5. | ALA3239782 | B | Activation of Nrf2 in rat PC12 cells assessed as increase of NQO-1 mRNA level at 1 to 4 uM after 4.5 to 9 hrs by qRT-PCR analysis | Rattus norvegicus | 2 | ALA3232949 | cell-based format | Scientific Literature | |
6. | ALA3239783 | B | Induction of nuclear translocation of Nrf2 in rat PC12 cells at 4 uM after 1.5 hrs by Western blot analysis | Rattus norvegicus | 1 | ALA3232949 | cell-based format | Scientific Literature | |
7. | ALA3239784 | B | Induction of nuclear translocation of Nrf2 in rat PC12 cells at 4 uM after 4.5 hrs by Western blot analysis | Rattus norvegicus | 1 | ALA3232949 | cell-based format | Scientific Literature | |
8. | ALA3419139 | B | Inhibition of Vps75-stimulated recombinant Saccharomyces cerevisiae histone acetyltransferase Rtt109 using Asf1-dH3-H4 as substrate assessed as coenzyme A production after 45 mins by CPM based HTS assay | Saccharomyces cerevisiae | 107 | ALA3414544 | single protein format | Scientific Literature | |
9. | ALA3419140 | B | Inhibition of Vps75-stimulated recombinant Saccharomyces cerevisiae histone acetyltransferase Rtt109 using Asf1-dH3-H4 as substrate assessed as coenzyme A production after 45 mins by CoA-based HTS counter screen assay | Saccharomyces cerevisiae | 104 | ALA3414544 | single protein format | Scientific Literature | |
10. | ALA3419141 | B | Inhibition of Vps75-stimulated recombinant Saccharomyces cerevisiae histone acetyltransferase Rtt109 assessed as H3K56 acetylated product at 125 uM after 45 mins by Slot bolt inhibiton assay | Saccharomyces cerevisiae | 104 | ALA3414544 | single protein format | Scientific Literature | |
11. | ALA3419142 | B | Inhibition of Vps75-stimulated recombinant Saccharomyces cerevisiae histone acetyltransferase Rtt109 assessed as H3K27 acetylated product at 125 uM after 45 mins by Slot bolt inhibiton assay | Saccharomyces cerevisiae | 104 | ALA3414544 | single protein format | Scientific Literature | |
12. | ALA3419143 | P | Fluorescence quenching assessed as fluorescence signal reduction at 10 uM after 10 mins | 104 | ALA3414544 | small-molecule physicochemical format | Scientific Literature | ||
13. | ALA3419144 | P | Fluorescence quenching in HTS buffer assessed as fluorescence signal reduction at 10 uM after 10 mins | 104 | ALA3414544 | small-molecule physicochemical format | Scientific Literature | ||
14. | ALA3419553 | P | Fluorescence quenching in HTS buffer with 7.5 uM CoA assessed as fluorescence signal reduction at 10 uM after 10 mins | 104 | ALA3414544 | small-molecule physicochemical format | Scientific Literature | ||
15. | ALA3419578 | P | Fluorescence quenching in HTS buffer with 20 uM 7-diethylamino-3-(4'-maleimidyl-phenyl)-4-methylcoumarin assessed as fluorescence signal reduction at 10 uM after 10 mins | 104 | ALA3414544 | small-molecule physicochemical format | Scientific Literature | ||
16. | ALA3620077 | B | Inhibition of Keap1 (unknown origin) interaction to Nrf2 | Homo sapiens | 8 | ALA3616400 | protein format | Scientific Literature | |
17. | ALA4376060 | B | Binding affinity to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) assessed as thermal stabilization by sypro orange dye based thermal shift assay | Homo sapiens | 19 | ALA4373719 | assay format | Scientific Literature | |
18. | ALA4376062 | B | Binding affinity to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) by surface plasmon resonance analysis | Homo sapiens | 21 | ALA4373719 | assay format | Scientific Literature | |
19. | ALA4376065 | B | Binding affinity to Keap1 in mouse Hepa1c1c7 cells assessed as reduction in Keap1 interaction by measuring induction of NQO1 activity at 1 to 10 uM measured after 24 hrs | Mus musculus | 10 | ALA4373719 | cell-based format | Scientific Literature | |
20. | ALA4376067 | B | Inhibition of Cys5-LDEETGEFL-NH2 binding to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) measured after 10 to 15 mins by fluorescence polarization assay | Homo sapiens | 25 | ALA4373719 | assay format | Scientific Literature |