| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3268102 | B | Inhibition of soluble human BACE1 (1 to 454) expressed in baculovirus infected insect Sf9 cells using EuK-KTEEISEVNLDAEFRHDKC-biotin as substrate preincubated for 30 mins followed by substrate addition measured after 3 hrs by FRET assay | Homo sapiens | 1 | ALA3259716 | cell-based format | Scientific Literature | |
| 2. | ALA3705045 | B | Time-Resolved FRET Assay: A homogeneous time-resolved FRET assay was used to determine IC50 values for inhibitors of the soluble human BACE1 catalytic domain. The assay monitored the increase of 620nm fluorescence that resulted from BACE1 cleavage of an APPswedish APPswe mutant peptide FRET substrate (QSY7-EISEVNLDAEFC-Europium-amide). | Homo sapiens | 43 | ALA3638861 | single protein format | BindingDB Database | |
| 3. | ALA3801549 | A | Inhibition of CYP3A4 in human liver microsomes using testosterone and midazolam as substrate preincubated for 30 mins followed substrate addition by LC-MS/MS analysis | Homo sapiens | 14 | ALA3797133 | tissue-based format | Scientific Literature | |
| 4. | ALA3801550 | B | Inhibition of recombinant human BACE1 preincubated for 30 mins followed QSY7EISEVNLDAEFC-Eu-amide substrate addition measured after 90 mins by FRET assay | Homo sapiens | 20 | ALA3797133 | single protein format | Scientific Literature | |
| 5. | ALA3801551 | B | Inhibition of BACE1 in human HEK293 cells transfected with human APP Swe/Lon mutations assessed as amyloid beta 40 level after 4 hrs by electrochemiluminescence-based sandwich ELISA | Homo sapiens | 18 | ALA3797133 | cell-based format | Scientific Literature | |
| 6. | ALA3801552 | B | Selectivity ratio, ratio for inhibition for human cathepsin D to inhibition for human BACE1 | Homo sapiens | 20 | ALA3797133 | assay format | Scientific Literature | |
| 7. | ALA3803621 | A | Drug uptake in rat brain homogenate at 10 mg/kg, po measured after 6 hrs | Rattus norvegicus | 9 | ALA3797133 | organism-based format | Scientific Literature | |
| 8. | ALA3803622 | A | Ratio of drug uptake in rat brain to plasma at 10 mg/kg, po measured after 6 hrs | Rattus norvegicus | 9 | ALA3797133 | organism-based format | Scientific Literature |
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