#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3268497	B	Displacement of [9-methyl-3H]BRL-43694 from human 5-HT3A receptor after overnight incubation by scintillation proximity assay	Homo sapiens	11	ALA3259590	single protein format	Scientific Literature
2.	ALA3268498	B	Partial agonist activity at human 5-HT3A receptor expressed in HEK293 cells assessed as change in Ca2+ level measured as intrinsic activity at 1 uM relative to 5-HT in presence of carbachol	Homo sapiens	6	ALA3259590	cell-based format	Scientific Literature
3.	ALA3268499	B	Partial agonist activity at mouse 5-HT3A receptor expressed in HEK293 cells assessed as change in Ca2+ level measured as intrinsic activity at 1 uM relative to 5-HT in presence of carbachol	Mus musculus	4	ALA3259590	cell-based format	Scientific Literature
4.	ALA3268500	B	Agonist activity at human 5-HT3A receptor expressed in HEK293 cells in presence of carbachol	Homo sapiens	6	ALA3259590	cell-based format	Scientific Literature
5.	ALA3268501	B	Agonist activity at mouse 5-HT3A receptor expressed in HEK293 cells in presence of carbachol	Mus musculus	4	ALA3259590	cell-based format	Scientific Literature
6.	ALA3268502	A	Inhibition of CYP1A2 (unknown origin)	Homo sapiens	7	ALA3259590	single protein format	Scientific Literature
7.	ALA3268503	A	Inhibition of CYP2B6 (unknown origin)	Homo sapiens	6	ALA3259590	single protein format	Scientific Literature
8.	ALA3268874	A	Inhibition of CYP2C9 (unknown origin)	Homo sapiens	6	ALA3259590	single protein format	Scientific Literature
9.	ALA3268875	A	Inhibition of CYP2C19 (unknown origin)	Homo sapiens	6	ALA3259590	single protein format	Scientific Literature
10.	ALA3268876	A	Inhibition of CYP2D6 (unknown origin)	Homo sapiens	6	ALA3259590	single protein format	Scientific Literature
11.	ALA3268877	A	Inhibition of CYP3A4 (unknown origin)	Homo sapiens	7	ALA3259590	single protein format	Scientific Literature
12.	ALA3268878	A	Intrinsic clearance in human liver microsomes	Homo sapiens	8	ALA3259590	assay format	Scientific Literature
13.	ALA3268879	F	Inhibition of 5-HT induced-bradycardia in ICR mouse von Bezold-Jarisch reflex model at 3 mg/kg, po after 60 mins relative to control	Mus musculus	1	ALA3259590	organism-based format	Scientific Literature
14.	ALA3706294	B	Radioligand Binding Assay: The relative affinity of the various compounds for the human 5-HT3 receptor was measured in a radioligand binding assay, using a scintillation proximity assay (SPA) format. Test compounds were dissolved to 10 mM in 100% DMSO, then serially diluted at 10x assay concentrations in 100% DMSO in 96-well polypropylene plates and further diluted to 4x assay concentrations with the assay buffer. Samples were incubated in 50 mM Tris-HCl, pH 7.5, 3 mM MgCl2, 1 mM EDTA and 10% DMSO with 10 nM [9-methyl-3H]BRL-43694 (Perkin Elmer), 3 ug of human 5-HT3 receptor membranes (Perkin Elmer) and 0.5 mg/mL SPA beads (WGA PVT, Amersham Biosciences) in a final volume of 0.2 mL. Binding reactions were set up in wells of PicoPlates-96 (Perkin Elmer) by adding consecutively 50 uL of each competing compound or buffer, SPA beads, the radioligand and 5-HT3 receptor membranes. After an overnight incubation at room temperature on a Nutator mixer, plates were centrifuged for 15 min at 1,500 rpm.	Homo sapiens	26	ALA3638654	assay format	BindingDB Database