# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3266742 | B | Inhibition of GGDPS in human RPMI-8226 cells assessed as disruption of Rap1a geranylgeranylation at 10 uM after 48 hrs by western blot analysis | Homo sapiens | 6 | ALA3259705 | cell-based format | Scientific Literature | |
2. | ALA3266744 | B | Inhibition of FDPS in human RPMI-8226 cells assessed as disruption of Ras farnesylation after 48 hrs by western blot analysis | Homo sapiens | 6 | ALA3259705 | cell-based format | Scientific Literature | |
3. | ALA3268700 | B | Inhibition of GGDPS in human RPMI-8226 cells assessed as decrease in intracellular GGPP level by measuring GGTase-1-mediated GGPP incorporation into fluorescent GCVLL peptide at 1 uM after 24 hrs by reverse phase HPLC analysis | Homo sapiens | 3 | ALA3259705 | cell-based format | Scientific Literature | |
4. | ALA3268701 | B | Inhibition of GGDPS in human RPMI-8226 cells assessed as increase in intracellular FPP level by measuring FTase-1-mediated FPP incorporation into fluorescent GCVLS peptide at 1 uM after 24 hrs by reverse phase HPLC analysis | Homo sapiens | 3 | ALA3259705 | cell-based format | Scientific Literature | |
5. | ALA3268702 | B | Inhibition of recombinant GGDPS (unknown origin) assessed as decrease in radiolabeld GGPP level using FPP and [14C]IPP as substrate treated with enzyme for 10 mins prior to substrate challenge for 30 mins by liquid scintillation counting analysis | Homo sapiens | 6 | ALA3259705 | single protein format | Scientific Literature | |
6. | ALA3268703 | B | Inhibition of recombinant FDPS (unknown origin) assessed as decrease in radiolabeld GGPP level using GPP and [14C]IPP as substrate treated with enzyme for 10 mins prior to substrate challenge for 30 mins by liquid scintillation counting analysis | Homo sapiens | 5 | ALA3259705 | single protein format | Scientific Literature | |
7. | ALA3268704 | B | Inhibition of recombinant FTase (unknown origin) assessed as decrease in prenylated fluorescent peptides level using dansyl-GCVLS and FPP as substrate after 1 hr by HPLC-fluorescence detection analysis | Homo sapiens | 5 | ALA3259705 | protein complex format | Scientific Literature | |
8. | ALA3268705 | B | Inhibition of recombinant GGTase 1 (unknown origin) assessed as decrease in prenylated fluorescent peptides level using dansyl-GCVLS and GGPP as substrate after 1 hr by HPLC-fluorescence detection analysis | Homo sapiens | 5 | ALA3259705 | assay format | Scientific Literature | |
9. | ALA3268706 | B | Inhibition of recombinant GGTase 2 (unknown origin) using recombinant Rab1A and [3H]GGPP as substrate after 20 mins by liquid scintillation counting analysis in presence of recombinant REP-2 | Homo sapiens | 5 | ALA3259705 | assay format | Scientific Literature | |
10. | ALA3268707 | B | Inhibition of GGDPS in human RPMI-8226 cells assessed as disruption of monoclonal protein trafficking by measuring accumulation of intracellular lambda light chain at 10 uM after 48 hrs by ELISA | Homo sapiens | 4 | ALA3259705 | cell-based format | Scientific Literature | |
11. | ALA3777120 | B | Inhibition of recombinant GGDPS (unknown origin) assessed as radiolabeled GGPP formation preincubated for 10 mins followed by addition of 10 uM FPP substrate and [14C]IPP for 30 mins by liquid scintillation counter analysis | Homo sapiens | 4 | ALA3774363 | single protein format | Scientific Literature | |
12. | ALA4813902 | B | Inhibition of recombinant GGDPS (unknown origin) using FPP and [14C] IPP as substrate preincubated for 10 mins followed by substrate addition measured after 30 mins by liquid scintillation counting method | Homo sapiens | 10 | ALA4811322 | single protein format | Scientific Literature | |
13. | ALA4813903 | B | Inhibition of GGDPS in human RPMI-8226 cells assessed as lowest effective concentration required to show unmodified Rap1a band after 48 hrs by immunoblot analysis | Homo sapiens | 8 | ALA4811322 | cell-based format | Scientific Literature |