#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3268036	Functional	Antiproliferative activity against human HeLa cells assessed as cell viability after 96 hrs by CellTiter96 assay	Homo sapiens	25	ALA3259640	cell-based format	Scientific Literature
2.	ALA3268037	Functional	Antiproliferative activity against human K562 cells assessed as cell viability after 96 hrs by CellTiter96 assay	Homo sapiens	25	ALA3259640	cell-based format	Scientific Literature
3.	ALA3268038	Functional	Antiproliferative activity against human MDA-MB-468 cells assessed as cell viability after 96 hrs by CellTiter96 assay	Homo sapiens	25	ALA3259640	cell-based format	Scientific Literature
4.	ALA3268039	Functional	Antiproliferative activity against human MDA-MB-231 cells assessed as cell viability after 96 hrs by CellTiter96 assay	Homo sapiens	25	ALA3259640	cell-based format	Scientific Literature
5.	ALA3268040	ADME	AUC in Sprague-Dawley rat at 2 mg/kg, iv compound formulated with 5% DMSO, 10% cremophor and 85% water by LC-MS/MS analysis	Rattus norvegicus	5	ALA3259640	organism-based format	Scientific Literature
6.	ALA3268041	ADME	Volume of distribution in Sprague-Dawley rat at 2 mg/kg, iv compound formulated with 5% DMSO, 10% cremophor and 85% water by LC-MS/MS analysis	Rattus norvegicus	5	ALA3259640	organism-based format	Scientific Literature
7.	ALA3268042	ADME	Clearance in Sprague-Dawley rat at 2 mg/kg, iv compound formulated with 5% DMSO, 10% cremophor and 85% water by LC-MS/MS analysis	Rattus norvegicus	5	ALA3259640	organism-based format	Scientific Literature
8.	ALA3268043	ADME	Oral bioavailability in Sprague-Dawley rat at 20 mg/kg compound formulated with 1% methylcellulose in water by LC-MS/MS analysis	Rattus norvegicus	5	ALA3259640	organism-based format	Scientific Literature
9.	ALA3268044	ADME	Oral bioavailability in Sprague-Dawley rat at 20 mg/kg compound formulated with 5% DMSO, 10% cremophor and 85% water by LC-MS/MS analysis	Rattus norvegicus	1	ALA3259640	organism-based format	Scientific Literature
10.	ALA3268045	Physicochemical	Solubility of the compound in phosphate buffered saline containing 1% DMSO at pH 7.4 after 2 hrs by HPLC analysis		2	ALA3259640	small-molecule physicochemical format	Scientific Literature
11.	ALA3268046	ADME	Half life in rat liver microsomes at 1 uM by LC-MS/MS analysis	Rattus norvegicus	2	ALA3259640	assay format	Scientific Literature
12.	ALA3268047	Functional	Antitumor activity against human MDA-MB-231 cells xenografted in BALB/c nu/nu mouse assessed as tumor volume at 20 mg/kg, iv qd for 28 days relative to control	Homo sapiens	1	ALA3259640	organism-based format	Scientific Literature
13.	ALA3268048	Functional	Antitumor activity against human MDA-MB-231 cells xenografted in BALB/c nu/nu mouse assessed as tumor volume at 150 mg/kg, po bid for 28 days administered via gavage relative to control	Homo sapiens	1	ALA3259640	organism-based format	Scientific Literature
14.	ALA3268049	Toxicity	Toxicity in BALB/c nu/nu mouse xenografted with human MDA-MB-231 cells assessed as body weight loss at 20 mg/kg, iv qd for 28 days	Mus musculus	1	ALA3259640	organism-based format	Scientific Literature
15.	ALA3268050	Toxicity	Toxicity in BALB/c nu/nu mouse xenografted with human MDA-MB-231 cells assessed as body weight loss at 150 mg/kg, po bid for 28 days	Mus musculus	1	ALA3259640	organism-based format	Scientific Literature
16.	ALA3268051	Binding	Inhibition of Hec1/Nek2 interaction in human K562 cells assessed as reduction in coimmunoprecipitated Hec1 level at 100 nM after 16 hrs by co-immunoprecipitation assay	Homo sapiens	1	ALA3259640	cell-based format	Scientific Literature
17.	ALA3268052	Binding	Inhibition of Hec1/Nek2 interaction in human K562 cells assessed as induction of Nek2 degradation after 24 hrs by western blot analysis	Homo sapiens	1	ALA3259640	cell-based format	Scientific Literature
18.	ALA3268053	Binding	Inhibition of Hec1/Nek2 interaction in human K562 cells assessed as induction of Nek2 degradation at 10 nM after 24 hrs by western blot analysis	Homo sapiens	1	ALA3259640	cell-based format	Scientific Literature
19.	ALA3268054	Binding	Inhibition of Hec1/Nek2 interaction in human MDA-MB-468 cells assessed as reduction in Hec1 level at 100 nM by western blot analysis	Homo sapiens	1	ALA3259640	cell-based format	Scientific Literature
20.	ALA3268055	Binding	Inhibition of Hec1/Nek2 interaction in human MDA-MB-468 cells assessed as induction of Nek2 degradation at 100 nM by western blot analysis	Homo sapiens	1	ALA3259640	cell-based format	Scientific Literature