# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3379759 | B | Allosteric antagonist activity at wild type LFA-1 (unknown origin) transfected in human K562 cells assessed as inhibition of cell adhesion to ICAM-1 at 50 uM incubated for 30 mins under dark condition by microplate fluorimetric analysis | Homo sapiens | 4 | ALA3297711 | cell-based format | Scientific Literature | |
2. | ALA3379760 | B | Antagonist activity at C-terminal 6xHis-tagged LFA-1 LBD (unknown origin) expressed in Escherichia coli assessed as inhibition of interaction with ICAM-1 preincubated for 30 mins followed by ICAM-1 addition measured after 60 mins by ELISA | Homo sapiens | 16 | ALA3297711 | assay format | Scientific Literature | |
3. | ALA3379761 | B | Antagonist activity at wild type LFA-1 in human Jurkat cells assessed as inhibition of PMA-stimulated cell adhesion to ICAM-1 at 50 uM incubated for 45 mins under dark condition by microplate fluorimetric analysis | Homo sapiens | 4 | ALA3297711 | cell-based format | Scientific Literature | |
4. | ALA3379762 | B | Allosteric antagonist activity at LFA-1 K287C/K294C double mutant (unknown origin) transfected in human K562 cells assessed as inhibition of cell adhesion to ICAM-1 at 50 uM incubated for 30 mins under dark condition by microplate fluorimetric analysis in absence of DTT | Homo sapiens | 4 | ALA3297711 | cell-based format | Scientific Literature | |
5. | ALA3379763 | B | Allosteric antagonist activity at LFA-1 K287C/K294C double mutant (unknown origin) transfected in human K562 cells assessed as inhibition of cell adhesion to ICAM-1 at 50 uM incubated for 30 mins under dark condition by microplate fluorimetric analysis in presence of DTT | Homo sapiens | 4 | ALA3297711 | cell-based format | Scientific Literature |