#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3378730	P	Retention time of the compound in 50 to 100% ACN/Water/0.1%TFA by RP-HPLC analysis		34		small-molecule physicochemical format	Scientific Literature
2.	ALA3378733	B	Competitive binding affinity to human full length recombinant GST-tagged eIF4E expressed in Escherichia coli BL21(DE3) assessed as inhibition of eIF4E/eIF4G interaction by fluorescence polarization assay relative to parent compound	Homo sapiens	54		assay format	Scientific Literature
3.	ALA4822124	B	Displacement of KKQYDREFLLDFQFK-FITCH from recombinant GST-tagged human eIF4E expressed in Escherichia coli BL21 (DE3) cells by fluorescence polarization analysis	Homo sapiens	2		cell-based format	Scientific Literature
4.	ALA4822125	F	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 24 hrs by Cell specific bioluminescence imaging (CS-BLI) assay	Homo sapiens	2		cell-based format	Scientific Literature
5.	ALA4822126	F	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by Cell specific bioluminescence imaging (CS-BLI) assay	Homo sapiens	2		cell-based format	Scientific Literature
6.	ALA4822127	F	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by Cell specific bioluminescence imaging (CS-BLI) assay	Homo sapiens	2		cell-based format	Scientific Literature
7.	ALA4822128	F	Cytotoxicity against human MM1.S cells assessed as reduction in cell viability after 24 hrs by Cell specific bioluminescence imaging (CS-BLI) assay	Homo sapiens	2		cell-based format	Scientific Literature
8.	ALA4822129	F	Cytotoxicity against human MM1.S cells assessed as reduction in cell viability after 48 hrs by Cell specific bioluminescence imaging (CS-BLI) assay	Homo sapiens	2		cell-based format	Scientific Literature
9.	ALA4822130	F	Cytotoxicity against human MM1.S cells assessed as reduction in cell viability after 72 hrs by Cell specific bioluminescence imaging (CS-BLI) assay	Homo sapiens	2		cell-based format	Scientific Literature
10.	ALA4822131	B	Inhibition of eIF4E in human HEK293T cells transfected with bicistronic transcript containing 5'-Firefly luciferase followed by IRES and 3'-Renilla luciferase assessed as inhibition of cap-dependent translation by measuring reduction in Firefly to Renilla luciferase activity ratio measured after 3 hrs by dual luciferase reporter assay	Homo sapiens	2		cell-based format	Scientific Literature
11.	ALA4822132	B	Disruption of eIF4E-eIF4G interaction in human HeLa cells at 6.25 to 100 uM measured after 1 hr by m7-GDP functionalized agarose beads pull down assay based western blot analysis	Homo sapiens	1		cell-based format	Scientific Literature
12.	ALA4822133	B	Potentiation of eIF4E-4EBP1 interaction in human HeLa cells at 6.25 to 100 uM measured after 1 hr by m7-GDP functionalized agarose beads pull down assay based western blot analysis	Homo sapiens	1		cell-based format	Scientific Literature
13.	ALA4822140	F	Ratio of IC50 for cytotoxicity against human MCF7 cells measured after 24 hrs to IC50 for cytotoxicity against human MCF7 cells measured after 72 hrs by Cell specific bioluminescence imaging (CS-BLI) assay	Homo sapiens	2		cell-based format	Scientific Literature
14.	ALA4822145	B	Binding affinity to 15N-labeled-GB1-eIF4E (unknown origin) at 50 to 300 uM measured by NMR analysis	Homo sapiens	2		single protein format	Scientific Literature