# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3378730 | P | Retention time of the compound in 50 to 100% ACN/Water/0.1%TFA by RP-HPLC analysis | 34 | ALA3351917 | small-molecule physicochemical format | Scientific Literature | ||
2. | ALA3378733 | B | Competitive binding affinity to human full length recombinant GST-tagged eIF4E expressed in Escherichia coli BL21(DE3) assessed as inhibition of eIF4E/eIF4G interaction by fluorescence polarization assay relative to parent compound | Homo sapiens | 54 | ALA3351917 | assay format | Scientific Literature | |
3. | ALA4822124 | B | Displacement of KKQYDREFLLDFQFK-FITCH from recombinant GST-tagged human eIF4E expressed in Escherichia coli BL21 (DE3) cells by fluorescence polarization analysis | Homo sapiens | 2 | ALA4819099 | cell-based format | Scientific Literature | |
4. | ALA4822125 | F | Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 24 hrs by Cell specific bioluminescence imaging (CS-BLI) assay | Homo sapiens | 2 | ALA4819099 | cell-based format | Scientific Literature | |
5. | ALA4822126 | F | Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by Cell specific bioluminescence imaging (CS-BLI) assay | Homo sapiens | 2 | ALA4819099 | cell-based format | Scientific Literature | |
6. | ALA4822127 | F | Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by Cell specific bioluminescence imaging (CS-BLI) assay | Homo sapiens | 2 | ALA4819099 | cell-based format | Scientific Literature | |
7. | ALA4822128 | F | Cytotoxicity against human MM1.S cells assessed as reduction in cell viability after 24 hrs by Cell specific bioluminescence imaging (CS-BLI) assay | Homo sapiens | 2 | ALA4819099 | cell-based format | Scientific Literature | |
8. | ALA4822129 | F | Cytotoxicity against human MM1.S cells assessed as reduction in cell viability after 48 hrs by Cell specific bioluminescence imaging (CS-BLI) assay | Homo sapiens | 2 | ALA4819099 | cell-based format | Scientific Literature | |
9. | ALA4822130 | F | Cytotoxicity against human MM1.S cells assessed as reduction in cell viability after 72 hrs by Cell specific bioluminescence imaging (CS-BLI) assay | Homo sapiens | 2 | ALA4819099 | cell-based format | Scientific Literature | |
10. | ALA4822131 | B | Inhibition of eIF4E in human HEK293T cells transfected with bicistronic transcript containing 5'-Firefly luciferase followed by IRES and 3'-Renilla luciferase assessed as inhibition of cap-dependent translation by measuring reduction in Firefly to Renilla luciferase activity ratio measured after 3 hrs by dual luciferase reporter assay | Homo sapiens | 2 | ALA4819099 | cell-based format | Scientific Literature | |
11. | ALA4822132 | B | Disruption of eIF4E-eIF4G interaction in human HeLa cells at 6.25 to 100 uM measured after 1 hr by m7-GDP functionalized agarose beads pull down assay based western blot analysis | Homo sapiens | 1 | ALA4819099 | cell-based format | Scientific Literature | |
12. | ALA4822133 | B | Potentiation of eIF4E-4EBP1 interaction in human HeLa cells at 6.25 to 100 uM measured after 1 hr by m7-GDP functionalized agarose beads pull down assay based western blot analysis | Homo sapiens | 1 | ALA4819099 | cell-based format | Scientific Literature | |
13. | ALA4822140 | F | Ratio of IC50 for cytotoxicity against human MCF7 cells measured after 24 hrs to IC50 for cytotoxicity against human MCF7 cells measured after 72 hrs by Cell specific bioluminescence imaging (CS-BLI) assay | Homo sapiens | 2 | ALA4819099 | cell-based format | Scientific Literature | |
14. | ALA4822145 | B | Binding affinity to 15N-labeled-GB1-eIF4E (unknown origin) at 50 to 300 uM measured by NMR analysis | Homo sapiens | 2 | ALA4819099 | single protein format | Scientific Literature |