| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3608038 | Binding | Binding affinity to eIF4E (unknown origin) by fluorescence quenching assay | Homo sapiens | 11 | ALA3603763 | single protein format | Scientific Literature | |
| 2. | ALA3608039 | ADME | Stability of the compound assessed as human DcpS-mediated compound hydrolysis at 40 uM after 10 to 180 mins by HPLC analysis | Homo sapiens | 11 | ALA3603763 | single protein format | Scientific Literature | |
| 3. | ALA3608043 | Binding | Inhibition of eIF4E-mediated translation in flexi rabbit reticulocyte lysate using luciferase mRNA treated simultaneously with mRNA by luminescence based luciferase assay | Oryctolagus cuniculus | 11 | ALA3603763 | assay format | Scientific Literature | |
| 4. | ALA3608168 | Binding | Inhibition of eIF4E-mediated translation in flexi rabbit reticulocyte lysate using luciferase mRNA preincubated for 60 mins before mRNA addition by luminescence based luciferase assay | Oryctolagus cuniculus | 11 | ALA3603763 | assay format | Scientific Literature | |
| 5. | ALA1953936 | Binding | Binding affinity to recombinant eIF4E by fluorescence quenching titration method | 14 | ALA1949618 | single protein format | Scientific Literature | ||
| 6. | ALA1953940 | Binding | Binding affinity to human recombinant DcpS by fluorescence quenching titration method | Homo sapiens | 13 | ALA1949618 | single protein format | Scientific Literature | |
| 7. | ALA1953941 | Binding | Selectivity ratio of Ka for human recombinant DcpS to Ka for eIF4E | Homo sapiens | 9 | ALA1949618 | assay format | Scientific Literature | |
| 8. | ALA1953942 | Binding | Inhibition of eIF4E-mediated mRNA translation in rabbit reticulocyte lysate by luciferase reporter gene assay | Oryctolagus cuniculus | 6 | ALA1949618 | assay format | Scientific Literature | |
| 9. | ALA1953943 | Binding | Inhibition of eIF4E-mediated mRNA translation in rabbit reticulocyte lysate pre-incubated for 60 mins prior to mRNA addition by luciferase reporter gene assay | Oryctolagus cuniculus | 6 | ALA1949618 | assay format | Scientific Literature | |
| 10. | ALA1953945 | Binding | Inhibition of eIF4E-mediated mRNA translation in rabbit reticulocyte lysate assessed as translation efficiency after 60 mins by luciferase reporter gene assay relative to m7GpppG-capped mRNA | Oryctolagus cuniculus | 12 | ALA1949618 | assay format | Scientific Literature | |
| 11. | ALA1023603 | Binding | Binding affinity to mouse eukaryotic initiation factor 4E expressed in Escherichia coli BL21(DE3) by fluorescence quenching titration method | Mus musculus | 6 | ALA1151839 | assay format | Scientific Literature | |
| 12. | ALA1023604 | ADME | Stability in presence of human DcpS assessed as complete degradation at 40 uM by HPLC-based assay | 6 | ALA1151839 | assay format | Scientific Literature | ||
| 13. | ALA1023605 | Binding | Inhibition of eukaryotic initiation factor 4E in micrococcal nuclease-pretreated rabbit reticulocyte lysate assessed as inhibition of m7,3'-O-GpppG-capped 5'UTRbetaglob-LUC mRNA translation by luciferase-based luminometry | Oryctolagus cuniculus | 6 | ALA1151839 | assay format | Scientific Literature | |
| 14. | ALA1023606 | Binding | Inhibition of eukaryotic initiation factor 4E in micrococcal nuclease-pretreated rabbit reticulocyte lysate assessed as inhibition of m7,3'-O-GpppG-capped 5'UTRbetaglob-LUC mRNA translation treated 60 mins before mRNA addition by luciferase-based luminometry | Oryctolagus cuniculus | 6 | ALA1151839 | assay format | Scientific Literature | |
| 15. | ALA1023607 | Binding | Inhibition of eukaryotic initiation factor 4E in micrococcal nuclease-pretreated rabbit reticulocyte lysate assessed as inhibition of rabbit beta-globulin mRNA translation by [3H]leucine incorporation assay | Oryctolagus cuniculus | 4 | ALA1151839 | assay format | Scientific Literature | |
| 16. | ALA2399732 | Physicochemical | Drug degradation in aqueous buffer at pH 2 after 4.5 hrs by RP HPLC analysis | 2 | ALA2396523 | small-molecule physicochemical format | Scientific Literature | ||
| 17. | ALA2399736 | Binding | Binding affinity to mouse eIF4E by fluorescence quenching assay | Mus musculus | 6 | ALA2396523 | single protein format | Scientific Literature | |
| 18. | ALA3992399 | Binding | Inhibition of recombinant flag-tagged DcpS (unknown origin) decapping activity assessed as reduction in hydrolysis of m7GpppG to m7G using unlabeled cap structure as substrate after 30 secs by PEI cellulose thin-layer chromatographic assay | Homo sapiens | 9 | ALA3991949 | single protein format | Patent Bioactivity Data | |
| 19. | ALA3992422 | Binding | Inhibition of recombinant flag-tagged DcpS (unknown origin) decapping activity assessed as hydrolysis of m7GpppG to m7G at 1.6 nM using unlabeled cap structure as substrate after 30 secs by PEI cellulose thin-layer chromatographic assay (Rvb = 62%) | Homo sapiens | 1 | ALA3991949 | single protein format | Patent Bioactivity Data | |
| 20. | ALA3992423 | Binding | Inhibition of recombinant flag-tagged DcpS (unknown origin) decapping activity assessed as hydrolysis of m7GpppG to m7G at 8 nM using unlabeled cap structure as substrate after 30 secs by PEI cellulose thin-layer chromatographic assay (Rvb = 62%) | Homo sapiens | 1 | ALA3991949 | single protein format | Patent Bioactivity Data |
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