| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3413597 | B | Inhibition of Topo 1 (unknown origin)-mediated relaxation of supercoiled pBR322 plasmid at 25 uM after 30 mins by agarose gel electrophoresis | Homo sapiens | 14 | ALA3407378 | single protein format | Scientific Literature | |
| 2. | ALA3413598 | B | Inhibition of human Topo 2-mediated relaxation of supercoiled pBR322 plasmid at 25 to 50 uM after 30 mins by agarose gel electrophoresis | Homo sapiens | 6 | ALA3407378 | single protein format | Scientific Literature | |
| 3. | ALA3413600 | F | Cytotoxicity against human HL60 cells after 48 hrs by MTT assay | Homo sapiens | 28 | ALA3407378 | cell-based format | Scientific Literature | |
| 4. | ALA3413601 | F | Cytotoxicity against human K562 cells after 48 hrs by MTT assay | Homo sapiens | 28 | ALA3407378 | cell-based format | Scientific Literature | |
| 5. | ALA3413602 | F | Cytotoxicity against human HeLa cells after 48 hrs by MTT assay | Homo sapiens | 28 | ALA3407378 | cell-based format | Scientific Literature | |
| 6. | ALA3413603 | F | Cytotoxicity against human A549 cells after 48 hrs by MTT assay | Homo sapiens | 28 | ALA3407378 | cell-based format | Scientific Literature | |
| 7. | ALA3413605 | B | Induction of calf thymus Topo 1-pBR322 DNA cleavage complex formation assessed as nicked DNA formation at 0.5 to 10 uM after 30 mins by optical density analysis | Bos taurus | 1 | ALA3407378 | single protein format | Scientific Literature | |
| 8. | ALA3413606 | B | Induction of calf thymus Topo 1-pBR322 DNA cleavage complex formation assessed as nicked DNA formation at 10 uM after 30 mins by optical density analysis relative to control | Bos taurus | 1 | ALA3407378 | single protein format | Scientific Literature | |
| 9. | ALA3413607 | B | Induction of calf thymus Topo 1-pBR322 DNA cleavage complex formation assessed as nicked DNA formation at 25 to 50 uM after 30 mins by optical density analysis relative to control | Bos taurus | 1 | ALA3407378 | single protein format | Scientific Literature | |
| 10. | ALA3413608 | B | Induction of Topo 1-DNA cleavage complex formation in human HL60 cells after 1 hr by ICE assay | Homo sapiens | 2 | ALA3407378 | cell-based format | Scientific Literature | |
| 11. | ALA3413609 | B | Induction of human Topo 2-pBR322 DNA cleavage complex formation assessed as linear DNA formation at 10 to 50 uM by agarose gel electrophoresis | Homo sapiens | 1 | ALA3407378 | single protein format | Scientific Literature | |
| 12. | ALA3413745 | B | Binding affinity to Escherichia coli pBR322 DNA assessed as DNA intercalation activity compound preincubated at 5 to 100 uM for 10 mins before enzyme addition by DNA unwinding assay in presence of topoisomerase 1 | Escherichia coli | 1 | ALA3407378 | nucleic acid format | Scientific Literature | |
| 13. | ALA3413746 | B | Binding affinity to calf thymus DNA at 15 uM after 5 mins by UV-vis spectrometry | Bos taurus | 1 | ALA3407378 | nucleic acid format | Scientific Literature |
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