Inhibition of HIV1 recombinant reverse transcriptase associated catalytically active RNase H domain assessed as reduction in DNA 3' end directed cleavage using HTS-2 RNA/DNA duplex substrate by fluorescence assay
Inhibition of HIV1 recombinant reverse transcriptase associated catalytically active RNase H domain assessed as reduction in RNA 5' end directed cleavage using HTS-3 RNA/DNA duplex substrate by fluorescence assay
Inhibition of HIV1 recombinant reverse transcriptase polymerase activity using [3H]TTP and poly(rA)-oligo(dT)16 substrate incubated for 20 mins by liquid scintillation spectrometry
Inhibition of HIV1 reverse transcriptase Y181C mutant associated RNase H domain activity assessed as reduction in internal cleavage using HTS-1 RNA/DNA duplex substrate by fluorescence assay
Inhibition of HIV1 reverse transcriptase Y181C mutant associated RNase H domain activity assessed as reduction in reduction in DNA 3' end directed cleavage using HTS-1 RNA/DNA duplex substrate by fluorescence assay
Inhibition of HIV1 reverse transcriptase Y181C mutant polymerase activity using [3H]TTP and poly(rA)-oligo(dT)16 substrate incubated for 20 mins by liquid scintillation spectrometry
Inhibition of HIV1 reverse transcriptase L100I/K103N mutant associated RNase H domain activity assessed as reduction in internal cleavage using HTS-1 RNA/DNA duplex substrate by fluorescence assay
Inhibition of HIV1 reverse transcriptase L100I/K103N mutant associated RNase H domain activity assessed as reduction in reduction in DNA 3' end directed cleavage using HTS-1 RNA/DNA duplex substrate by fluorescence assay
Inhibition of HIV1 reverse transcriptase L100I/K103N mutant polymerase activity using [3H]TTP and poly(rA)-oligo(dT)16 substrate incubated for 20 mins by liquid scintillation spectrometry
Inhibition of Moloney murine leukemia virus reverse transcriptase associated RNase H pre-incubated for 10 mins before HTS-1 RNA/DNA duplex substrate addition by fluorescence assay
Inhibition of Moloney murine leukemia virus reverse transcriptase polymerase activity using Td100/Pd18 DNA-DNA substrate incubated for 30 mins by fluorescence assay
Selectivity index, ratio of IC50 for HIV integrase to IC50 for HIV1 recombinant reverse transcriptase associated catalytically active RNase H-mediated domain internal cleavage activity
Antiviral activity against HIV infected in human HeLa P4/R5 cells pre-incubated for 16 hrs before virus infection and measured 48 hrs post virus infection by fluorescence-based beta-galactosidase detection assay
Inhibition of HIV1 recombinant reverse transcriptase associated catalytically active RNase H domain assessed as reduction in internal cleavage using HTS-1 RNA/DNA duplex substrate by fluorescence assay