#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3420275	B	Inhibition of HDAC6 in human A549 cells assessed as induction of tubulin acetylation after 17 to 18 hrs by ELISA	Homo sapiens	27		cell-based format	Scientific Literature
2.	ALA3420276	B	Inhibition of HDAC1 (unknown origin) using RHKK(Ac)AMC as substrate	Homo sapiens	27		single protein format	Scientific Literature
3.	ALA3420277	B	Inhibition of HDAC6 (unknown origin) using RHKK(Ac)AMC as substrate	Homo sapiens	27		single protein format	Scientific Literature
4.	ALA3420550	B	Inhibition of HDAC8 (unknown origin) using RHK(Ac)K(Ac)AMC as substrate	Homo sapiens	27		single protein format	Scientific Literature
5.	ALA3420551	P	Solubility of the compound in PBS measured after overnight incubation by LYSA assay		27		small-molecule physicochemical format	Scientific Literature
6.	ALA3420554	B	Inhibition of HDAC1/HDAC2/HDAC3 in human HeLa cells assessed as induction of p21 gene expression at 10 uM after 15 hrs by luciferase reporter gene assay relative to HDAC1 inhibitor SNDX-275	Homo sapiens	8		cell-based format	Scientific Literature
7.	ALA3420558	A	Plasma clearance in ICR mouse	Mus musculus	6		organism-based format	Scientific Literature
8.	ALA3706210	B	Fluorometric Assay: Compounds were tested for their ability to inhibit histone deacetylase 8 using an in vitro deacetylation assay. In a detailed procedure, 8 μl of HDAC8 enzyme solution (0.125 μg/μl) was transferred to assay plates (CORNING 3676). 1 μl of half-log diluted compounds were added into wells and incubated for 15 min at rt. After that, 8 μl of substrate solution was transferred to the wells and incubated for 30 min at rt. After deacetylation of the substrate by incubation with HDAC8 enzyme, subsequent exposure to a developing reagent produced a fluorophore that was directly proportional to the level of deacetylation. So 4 μl of developer regent (Cayman 10006394) was added into the wells in the assay plates and incubated for 15 min. The fluorescence signal was measured by a FlexStation3 plate reader (excitation wave length 340-360 nm; emission wave length 440-465 nm).	Homo sapiens	65		single protein format	BindingDB Database