# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3420628 | Binding | Inhibition of human recombinant 5-LOX expressed in Escherichia coli BL21 incubated for 10 mins in presence of arachidonic acid by RP-HPLC based cell-free assay | Homo sapiens | 49 | ALA3414543 | assay format | Scientific Literature | |
2. | ALA3420629 | Binding | Inhibition of 5-LOX in A23187-stimulated human blood PMNL assessed as reduction in lipoxygenase products formation pre-incubated for 15 mins followed by stimulation with A23187 for 10 mins by RP-HPLC based cell based assay | Homo sapiens | 49 | ALA3414543 | cell-based format | Scientific Literature | |
3. | ALA3420867 | Binding | Inhibition of 5-LOX in A23187-stimulated human blood PMNL assessed as reduction in lipoxygenase products formation in presence of arachidonic acid pre-incubated for 15 mins followed by stimulation with A23187 for 10 mins by RP-HPLC based cell based assay | Homo sapiens | 49 | ALA3414543 | cell-based format | Scientific Literature | |
4. | ALA3420869 | Binding | Selectivity index, ratio of inhibition for 12-LOX in human blood PMNL to IC50 for 5-LOX in human blood PMNL | Homo sapiens | 4 | ALA3414543 | assay format | Scientific Literature | |
5. | ALA3420870 | Binding | Selectivity index, ratio of inhibition for 12-LOX-1 in human blood PMNL to IC50 for 5-LOX in human blood PMNL | Homo sapiens | 4 | ALA3414543 | assay format | Scientific Literature | |
6. | ALA3420871 | Binding | Inhibition of mPGES1 in IL-1beta stimulated human A549 cell microsomal membranes assessed as reduction in PGE2 synthase activity after 15 mins using PGH2 substrate by RP-HPLC method | Homo sapiens | 50 | ALA3414543 | cell-based format | Scientific Literature | |
7. | ALA3420872 | Binding | Inhibition of 15-LOX-1 in human blood PMNL assessed as remaining activity by measuring 15-HETE synthesis at 10 uM | Homo sapiens | 50 | ALA3414543 | single protein format | Scientific Literature | |
8. | ALA3420874 | Binding | Inhibition of 12-LOX in human blood PMNL assessed as remaining activity by measuring 12-HETE synthesis at 10 uM | Homo sapiens | 50 | ALA3414543 | single protein format | Scientific Literature | |
9. | ALA3420875 | Binding | Inhibition of mPGES1 in IL-1beta stimulated human A549 cell microsomal membranes assessed as assessed as remaining PGE2 synthase activity at 10 uM after 15 mins using PGH2 substrate by RP-HPLC method | Homo sapiens | 32 | ALA3414543 | cell-based format | Scientific Literature | |
10. | ALA3994872 | Binding | Non-competitive inhibition of recombinant human 5-lipoxygenase expressed in Escherichia coli BL21 using varying levels of arachidonic acid as substrate preincubated for 15 mins followed by substrate addition measured after 10 mins by RP-HPLC method | Homo sapiens | 2 | ALA3994578 | assay format | Scientific Literature | |
11. | ALA3994873 | Functional | Anti-inflammatory activity in CD-1 mouse assessed as inhibition of carrageenan-induced paw edema at 1 mg/kg, ip pretreated for 30 mins followed by carrageenan injection measured after 4 hrs by hydroplethismometric method relative to control | Mus musculus | 2 | ALA3994578 | organism-based format | Scientific Literature | |
12. | ALA3994874 | Functional | Anti-inflammatory activity in CD-1 mouse assessed as inhibition of carrageenan-induced paw edema at 1 mg/kg, ip pretreated for 30 mins followed by carrageenan injection measured up to 48 hrs by hydroplethismometric method | Mus musculus | 1 | ALA3994578 | organism-based format | Scientific Literature | |
13. | ALA3994876 | Functional | Anti-inflammatory activity in CD-1 mouse model of Zymosan-induced peritonitis assessed as LTC4 level in peritoneal lavage at 1 mg/kg, ip pretreated for 30 mins followed by Zymosan injection measured after 30 mins by EIA (Rvb = 10.48 +/- 1.72 ng/ml) | Mus musculus | 2 | ALA3994578 | organism-based format | Scientific Literature | |
14. | ALA3994878 | Functional | Anti-inflammatory activity in CD-1 mouse model of Zymosan-induced peritonitis assessed as inflammatory cells in peritoneal lavage at 1 mg/kg, ip pretreated for 30 mins followed by Zymosan injection measured after 4 hrs (Rvb = 7 +/- 0.47 10'6No_unit) | Mus musculus | 2 | ALA3994578 | organism-based format | Scientific Literature |