# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3417877 | Binding | Displacement of FITC-BIM from recombinant N-terminal GST-tagged human BAX expressed in Escherichia coli after 30 mins by fluorescence polarization assay | Homo sapiens | 16 | ALA3414479 | assay format | Scientific Literature | |
2. | ALA3417878 | Binding | Displacement of FITC-BIM from recombinant human Bcl-2 expressed in Escherichia coli after 30 mins by fluorescence polarization assay | Homo sapiens | 16 | ALA3414479 | assay format | Scientific Literature | |
3. | ALA3417879 | Binding | Displacement of FITC-BIM from recombinant human Bcl-XL expressed in Escherichia coli after 30 mins by fluorescence polarization assay | Homo sapiens | 16 | ALA3414479 | assay format | Scientific Literature | |
4. | ALA3417880 | Functional | Induction of apoptosis in wild-type MEF assessed as appearance of fragmented nuclei and cell membrane blebbing after 24 hrs by DAPI staining | Mus musculus | 2 | ALA3414479 | cell-based format | Scientific Literature | |
5. | ALA3417881 | Functional | Induction of apoptosis in Bcl2-deficient MEF assessed as appearance of fragmented nuclei and cell membrane blebbing after 24 hrs by DAPI staining | Mus musculus | 2 | ALA3414479 | cell-based format | Scientific Literature | |
6. | ALA3417882 | Functional | Induction of apoptosis in Bad-deficient MEF assessed as appearance of fragmented nuclei and cell membrane blebbing after 24 hrs by DAPI staining | Mus musculus | 2 | ALA3414479 | cell-based format | Scientific Literature | |
7. | ALA3417883 | Functional | Induction of apoptosis in Bid-deficient MEF assessed as appearance of fragmented nuclei and cell membrane blebbing after 24 hrs by DAPI staining | Mus musculus | 2 | ALA3414479 | cell-based format | Scientific Literature | |
8. | ALA3417884 | Functional | Induction of apoptosis in Bak-deficient MEF assessed as appearance of fragmented nuclei and cell membrane blebbing after 24 hrs by DAPI staining | Mus musculus | 2 | ALA3414479 | cell-based format | Scientific Literature | |
9. | ALA3417885 | Functional | Induction of apoptosis in Bax-deficient MEF assessed as appearance of fragmented nuclei and cell membrane blebbing after 24 hrs by DAPI staining | Mus musculus | 2 | ALA3414479 | cell-based format | Scientific Literature | |
10. | ALA3420567 | Functional | Induction of apoptosis in Bax/Bak-deficient MEF assessed as appearance of fragmented nuclei and cell membrane blebbing after 24 hrs by DAPI staining | Mus musculus | 2 | ALA3414479 | cell-based format | Scientific Literature | |
11. | ALA3420575 | Functional | Induction of apoptosis in MEF assessed as appearance of cell membrane blebbing at 15 uM after 24 hrs by DAPI staining in presence of caspase inhibitor Ac-DEVD-CHO | Mus musculus | 1 | ALA3414479 | cell-based format | Scientific Literature | |
12. | ALA3420576 | Binding | Activation of Bax in human HuH7 cells assessed as inhibition of mitochondrial accumulation of fluorescent probe Mitotracker Red after 12 to 72 hrs by spectrophotometric analysis | Homo sapiens | 2 | ALA3414479 | cell-based format | Scientific Literature | |
13. | ALA3420578 | Binding | Activation of Bax in human HuH7 cells assessed as conformational changes by SDS-PAGE analysis | Homo sapiens | 1 | ALA3414479 | cell-based format | Scientific Literature | |
14. | ALA3420579 | Binding | Induction of Bax translocation from cytoplasm to mitochondria in human HuH7 cells at 10 uM after 12 hrs by Western blot analysis | Homo sapiens | 1 | ALA3414479 | cell-based format | Scientific Literature | |
15. | ALA3420580 | Binding | Activation of Bax in human HuH7 cells assessed as cyt-C release from mitochondria by MitoTracker Red dye-based immunofluorescence assay | Homo sapiens | 1 | ALA3414479 | cell-based format | Scientific Literature | |
16. | ALA3420581 | Binding | Activation of Bax in human HuH7 cells assessed as induction of caspase-3 activation at 10 uM by immunofluorescence assay | Homo sapiens | 1 | ALA3414479 | cell-based format | Scientific Literature | |
17. | ALA3420582 | Binding | Activation of Bax in human HuH7 cells assessed as formation of apoptotic nuclei at 10 uM by immunofluorescence assay | Homo sapiens | 1 | ALA3414479 | cell-based format | Scientific Literature | |
18. | ALA3420583 | Binding | Activation of Bax mutant in human Jurkat cells assessed as increase in apoptosis rate at 10 uM after 72 hrs by DAPI staining | Homo sapiens | 1 | ALA3414479 | cell-based format | Scientific Literature | |
19. | ALA3420584 | Binding | Activation of Bax in human NB4 cells assessed as increase in apoptosis rate at 10 uM after 12 hrs by DAPI staining | Homo sapiens | 1 | ALA3414479 | cell-based format | Scientific Literature | |
20. | ALA3420585 | ADME | Cytotoxicity against C57BL/6J mouse primary splenocytes assessed as effect on apoptosis rate at 10 uM after 72 hrs by DAPI staining | Mus musculus | 1 | ALA3414479 | cell-based format | Scientific Literature |