# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3429503 | Binding | Inhibition of C-terminal His6-tagged recombinant human membrane bound COMT expressed in Bacmid infected Sf-9 insect cells using dopamine/SAM as substrate/cofactor preincubated for 2 hrs followed by dopamine/SAM addition after 25 mins by fluorescence polarization assay | Homo sapiens | 26 | ALA3425416 | cell-based format | Scientific Literature | |
2. | ALA3429504 | Binding | Inhibition of recombinant rat membrane bound COMT expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL using dopamine/SAM as substrate/cofactor preincubated for 2 hrs followed by dopamine/SAM addition after 25 mins by fluorescence polarization assay | Rattus norvegicus | 13 | ALA3425416 | assay format | Scientific Literature | |
3. | ALA3429505 | Binding | Inhibition of recombinant human soluble COMT using dopamine/SAM as substrate/cofactor preincubated for 2 hrs followed by dopamine/SAM addition after 25 mins by fluorescence polarization assay | Homo sapiens | 13 | ALA3425416 | single protein format | Scientific Literature | |
4. | ALA3429506 | Binding | Selectivity ratio of IC50 for recombinant human soluble COMT to IC50 for C-terminal His6-tagged recombinant human membrane bound COMT | Homo sapiens | 9 | ALA3425416 | assay format | Scientific Literature |