# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3428784 | B | Binding affinity to 6His-tagged recombinant human NF-kappaB p50 N-terminal domain expressed in Escherichia coli BL21 (DE3) up to 10:1 compound to protein molar ratio after 2 hrs by mass spectrometric analysis | Homo sapiens | 5 | ALA3425410 | assay format | Scientific Literature | |
2. | ALA3428788 | B | Inhibition of TNF-alpha-stimulated NF-kappaB p50 (unknown origin) expressed in HEK293 cells at 1 to 100 uM preincubated for 4 hrs followed by TNF-alpha challenge measured after 24 hrs by secreted alkaline phosphatase reporter assay | Homo sapiens | 4 | ALA3425410 | cell-based format | Scientific Literature | |
3. | ALA3428789 | A | Cytotoxicity against human Jurkat T cells assessed as appearance of early apoptotic cells at 25 uM after 24 hrs by annexin V-FLUOS/propidium iodide staining-based flow cytometric analysis | Homo sapiens | 6 | ALA3425410 | cell-based format | Scientific Literature | |
4. | ALA3428790 | A | Cytotoxicity against human Jurkat T cells assessed as appearance of late apoptotic cells at 25 uM after 24 hrs by annexin V-FLUOS/propidium iodide staining-based flow cytometric analysis | Homo sapiens | 6 | ALA3425410 | cell-based format | Scientific Literature | |
5. | ALA3428791 | A | Cytotoxicity against human Jurkat T cells assessed as appearance of necrotic cells at 25 uM after 24 hrs by annexin V-FLUOS/propidium iodide staining-based flow cytometric analysis | Homo sapiens | 6 | ALA3425410 | cell-based format | Scientific Literature | |
6. | ALA3428792 | A | Cytotoxicity against human Jurkat T cells assessed as appearance of viable cells at 25 uM after 24 hrs by annexin V-FLUOS/propidium iodide staining-based flow cytometric analysis | Homo sapiens | 6 | ALA3425410 | cell-based format | Scientific Literature |