Activation of recombinant human pancreatic glucokinase using 10 mM glucose as substrate assessed as enzyme half maximal saturation concentration at 1 uM by G6PDH coupled assay
Activation of glucokinase in rat primary hepatocytes assessed as increase in 5.6 mM 2-deoxy-D-[3H]-glucose uptake after 4 hrs by scintillation counting analysis relative to control
Activation of glucokinase in 0 to 5.6 mM glucose-stimulated Sprague-Dawley rat pancreatic islets assessed as increase in insulin secretion after 1 hr by ELISA relative to control
Antidiabetic activity in C57BL/6 mouse assessed as reduction of blood glucose AUC at 50 mg/kg, po administered 30 mins prior to glucose challenge measured over 180 mins by OGTT relative to vehicle-treated control
Antidiabetic activity in C57BL/6 mouse assessed as reduction of blood glucose AUC at 30 mg/kg, po administered 30 mins prior to glucose challenge measured over 180 mins by OGTT relative to vehicle-treated control
Antidiabetic activity in po dosed C57BL/6J mouse assessed as blood glucose lowering effect administered 30 mins prior to glucose challenge measured over 180 mins by OGTT