# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3586822 | Binding | Inhibition of Sprague-Dawley rat brain microsomal FAAH using N-(2-hydroxyethyl)-4-pyren-1-ylbutanamide as substrate assessed as 4-pyren-1-ylbutanoic acid release preincubated for 10 mins with substrate followed by protein addition measured after 60 mins by reverse phase HPLC with fluorescence detection method | Rattus norvegicus | 23 | ALA3585341 | assay format | Scientific Literature | |
2. | ALA3587057 | Binding | Inhibition of human recombinant MAGL using 1,3-dihydroxypropan-1-yl 4-pyren-1-yl butanoate as substrate preincubated for 15 mins with substrate followed by protein addition measured after 45 mins by reverse phase HPLC with fluorescence detection method | Homo sapiens | 3 | ALA3585341 | single protein format | Scientific Literature | |
3. | ALA3587058 | Binding | Inhibition of porcine platelet cPLA2alpha using co-vesicles consisting 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphocholine and 1,2-dioleoyl-sn-glycerol as substrate assessed as arachidonic acid release at 10 uM after 60 mins by reverse phase HPLC with UV-detection method | Sus scrofa | 8 | ALA3585341 | assay format | Scientific Literature | |
4. | ALA3587059 | ADME | Metabolic stability in rat liver S9 fractions assessed as remaining compound after 30 mins by reverse phase HPLC with UV-detection method in presence of NADPH | Rattus norvegicus | 8 | ALA3585341 | subcellular format | Scientific Literature | |
5. | ALA3587060 | ADME | Metabolic stability in porcine plasma assessed as remaining compound at 5 mM after 30 mins by reverse phase HPLC with UV-detection method | Sus scrofa | 8 | ALA3585341 | cell-free format | Scientific Literature | |
6. | ALA3587061 | Binding | Selectivity ratio of IC50 for human recombinant MAGL to IC50 for Sprague-Dawley rat brain microsomal FAAH | 2 | ALA3585341 | assay format | Scientific Literature |