| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3587046 | B | Binding affinity to Fe(2) assessed as half maximal concentration required for binding 20 uM Fe(2) in pH 7.0 sodium phosphate buffer | 8 | ALA3585337 | assay format | Scientific Literature | ||
| 2. | ALA3587047 | B | Binding affinity to Fe(2) assessed as Fe(compound)2+/3 complex formation at 150 uM in Tris buffer at pH 7.8 at 30 degC | 4 | ALA3585337 | assay format | Scientific Literature | ||
| 3. | ALA3587048 | B | Inhibition of human human recombinant CP4H1 expressed in Escherichia coli Origami B(DE3) at 10 uM pre-incubated for 2 mins followed by alpha-ketoglutarate addition using dansylGlyProProGlyOEt substrate in Tris-HCl buffer at pH 7.8 at 30 degC by HPLC method | Homo sapiens | 5 | ALA3585337 | assay format | Scientific Literature | |
| 4. | ALA3587049 | B | Inhibition of human human recombinant CP4H1 expressed in Escherichia coli Origami B(DE3) pre-incubated for 2 mins followed by alpha-ketoglutarate addition using dansylGlyProProGlyOEt substrate in Tris-HCl buffer at pH 7.8 at 30 degC by HPLC method | Homo sapiens | 3 | ALA3585337 | assay format | Scientific Literature | |
| 5. | ALA3587050 | B | Inhibition of human human recombinant CP4H1 expressed in Escherichia coli Origami B(DE3) pre-incubated for 2 mins followed by alpha-ketoglutarate addition using dansylGlyProProGlyOEt substrate in Tris-HCl buffer at pH 7.8 at 30 degC by HPLC based Cheng-Prussoff equation method | Homo sapiens | 3 | ALA3585337 | assay format | Scientific Literature | |
| 6. | ALA3587051 | B | Competitive inhibition of human human recombinant CP4H1 expressed in Escherichia coli Origami B(DE3) pre-incubated for 2 mins followed by alpha-ketoglutarate addition using dansylGlyProProGlyOEt substrate in Tris-HCl buffer at pH 7.8 at 30 degC by HPLC based Lineweaver-Burk analysis | Homo sapiens | 2 | ALA3585337 | assay format | Scientific Literature | |
| 7. | ALA3587052 | B | Inhibition of N-terminal hexa-histidine tagged human PHD2 (181 to 426 amino acids) at 10 uM pre-incubated for 2 mins followed by alpha-ketoglutarate addition using HIF1alpha peptide (556 to 574 amino acids) and 50 uM FeSO4 by RP-HPLC method | Homo sapiens | 9 | ALA3585337 | single protein format | Scientific Literature |
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