#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3593134	B	Inhibition of MEK U911-activated ERK2 (unknown origin) using Erktide as substrate preincubated with enzyme for 20 mins prior to substrate addition by RapidFire Mass Spectrometry in presence of ATP at Km concentration	Homo sapiens	20		single protein format	Scientific Literature
2.	ALA3593135	B	Inhibition of MEK U911-activated ERK2 (unknown origin) using Erktide as substrate preincubated with enzyme for 20 mins prior to substrate addition by RapidFire Mass Spectrometry in presence of ATP at higher concentration	Homo sapiens	19		single protein format	Scientific Literature
3.	ALA3593136	B	Inhibition of ERK2 in human A375 cells harboring B-RAF V600E mutant assessed as decrease in phospho-ERK2 level after 2 hrs by Cellomics ArrayScanTM VTI imaging analysis	Homo sapiens	20		cell-based format	Scientific Literature
4.	ALA3593137	B	Inhibition of ERK1/2 in human A375 cells harboring B-RAF V600E mutant assessed as decrease in phospho-RSK level after 2 hrs by Cellomics ArrayScanTM VTI imaging analysis	Homo sapiens	20		cell-based format	Scientific Literature
5.	ALA3593138	F	Antiproliferative activity against human A375 cells harboring B-RAF V600E mutant after 72 hrs by Cellomics ArrayScanTM VTI imaging analysis	Homo sapiens	17		cell-based format	Scientific Literature
6.	ALA3593139	B	Inhibition of MEK1 (unknown origin) autophosphorylation incubated with enzyme for 20 mins prior to ATP addition by ADP Glo kinase assay in presence of ATP at Km concentration	Homo sapiens	17		single protein format	Scientific Literature
7.	ALA3593140	P	1-Octanol-aqueous phosphate buffer partition coefficient, logD of the compound at pH 7.4		17		small-molecule physicochemical format	Scientific Literature
8.	ALA3593141	P	Solubility of the compound in aqueous phosphate buffer at 25 degC at pH 7.4 after 24 hrs		17		small-molecule physicochemical format	Scientific Literature
9.	ALA3593142	B	Selectivity for ERK (unknown origin) over C-RAF (unknown origin) at 1 uM	Homo sapiens	11		assay format	Scientific Literature
10.	ALA3593143	B	Selectivity for ERK (unknown origin) over MEK1 (unknown origin) at 1 uM	Homo sapiens	8		assay format	Scientific Literature
11.	ALA3591260	A	Intrinsic clearance in human liver microsomes	Homo sapiens	8		assay format	Scientific Literature
12.	ALA3591261	A	Intrinsic clearance in rat hepatocytes	Rattus norvegicus	8		cell-based format	Scientific Literature
13.	ALA3591263	B	Irreversible inhibition of ERK1/2 in human Calu6 cells assessed as decrease in p90RSK phosphorylation at Thr359/Ser363 at 500 nM measured for up to 48 hrs after drug wash-out by immunoblot analysis	Homo sapiens	1		cell-based format	Scientific Literature
14.	ALA3591264	B	Inhibition of ERK1/2 in human A375 cells harboring B-RAF V600E mutant assessed as maximal decrease in p90RSK phosphorylation at Thr359/Ser363 measured between 20 and 30 hrs after drug treatment by immunoblot analysis	Homo sapiens	1		cell-based format	Scientific Literature
15.	ALA3591267	U	GSH reactivity assessed as half life		3		assay format	Scientific Literature
16.	ALA3591268	P	Stability in pH 7.4 buffer assessed as drug hydrolysis		3		small-molecule physicochemical format	Scientific Literature
17.	ALA3591270	B	Binding affinity to ERK2 in mouse fibroblasts assessed as half-life	Mus musculus	1		single protein format	Scientific Literature
18.	ALA3591271	B	Binding affinity to ERK1 in mouse fibroblasts assessed as half-life	Mus musculus	1		single protein format	Scientific Literature