# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3599997 | B | Inhibition of recombinant mouse RANKL-induced osteoclastogenesis of mouse RAW264.7 cells assessed as reduction of TRAP-positive multinucleated cells at 5 uM after 4 days by light microscopic analysis relative to control | Mus musculus | 12 | cell-based format | Scientific Literature | ||
2. | ALA3599998 | A | Cytotoxicity against mouse RAW264.7 cells assessed as cell viability at 5 uM after 24 hrs by MTT assay relative to control | Mus musculus | 12 | cell-based format | Scientific Literature | ||
3. | ALA3599999 | B | Inhibition of recombinant mouse RANKL-induced TRAP activity in mouse RAW264.7 cells using 4-nitrophenyl phosphate as TRAP substrate preincubated for 4 days followed by substrate addition measured after 15 mins by ELISA reader analysis | Mus musculus | 4 | cell-based format | Scientific Literature | ||
4. | ALA3600000 | B | Inhibition of recombinant mouse RANKL-induced osteoclastogenesis of mouse RAW264.7 cells assessed as suppression of NFATc1 mRNA expression at 2.5 to 5 uM preincubated for 1 hr followed by RANKL addition measured after 24 hrs by RT-PCR analysis | Mus musculus | 2 | cell-based format | Scientific Literature | ||
5. | ALA3600001 | B | Inhibition of recombinant mouse RANKL-induced osteoclastogenesis of mouse RAW264.7 cells assessed as suppression of c-fos mRNA expression at 2.5 to 5 uM preincubated for 1 hr followed by RANKL addition measured after 24 hrs by RT-PCR analysis | Mus musculus | 2 | cell-based format | Scientific Literature | ||
6. | ALA3600002 | B | Inhibition of recombinant mouse RANKL-induced osteoclastogenesis of mouse RAW264.7 cells assessed as suppression of TRAP mRNA expression at 2.5 to 5 uM preincubated for 1 hr followed by RANKL addition measured after 24 hrs by RT-PCR analysis | Mus musculus | 2 | cell-based format | Scientific Literature | ||
7. | ALA3600003 | B | Inhibition of recombinant mouse RANKL-induced osteoclastogenesis of mouse RAW264.7 cells assessed as suppression of cathepsin K mRNA expression at 2.5 to 5 uM preincubated for 1 hr followed by RANKL addition measured after 24 hrs by RT-PCR analysis | Mus musculus | 2 | cell-based format | Scientific Literature | ||
8. | ALA3600205 | B | Inhibition of recombinant mouse RANKL-induced NF-kappaB expression in nucleus of mouse RAW264.7 cells at 5 uM preincubated for 1 hr followed by RANKL addition measured after 24 hrs by ELISA plate analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
9. | ALA3600206 | B | Inhibition of recombinant mouse RANKL-induced NF-kappaB expression in nuclear extracts of mouse RAW264.7 cells assessed as decrease in ratio of NF-kappaB to TBP level at 5 uM preincubated for 1 hr followed by RANKL addition measured after 24 hrs by Western blot analysis relative to control | Mus musculus | 1 | assay format | Scientific Literature | ||
10. | ALA3600207 | B | Inhibition of recombinant mouse RANKL-induced NF-kappaB expression in nuclear extracts of mouse RAW264.7 cells assessed as decrease in ratio of NFATc1 to TBP level at 5 uM preincubated for 1 hr followed by RANKL addition measured after 24 hrs by Western blot analysis relative to control | Mus musculus | 1 | assay format | Scientific Literature | ||
11. | ALA3600208 | B | Inhibition of recombinant mouse RANKL-induced bone resorptive activity of mature osteoclasts derived from mouse RAW264.7 cells assessed as attenuation of resorbing pit formation on dentine slices after 4 days by toluidine blue staining assay | Mus musculus | 1 | tissue-based format | Scientific Literature |