#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3602876	B	Antagonist activity at XIAP-BIR3 domain (unknown origin) assessed as inhibition of interaction with SMAC by fluorescence polarization assay	Homo sapiens	14	ALA3600342	single protein format	Scientific Literature
2.	ALA3602884	F	Cytotoxicity against human sensitive EVSA-T cells assessed as inhibition of cell growth after 72 hrs by Alamar Blue assay	Homo sapiens	2	ALA3600342	cell-based format	Scientific Literature
3.	ALA3602885	F	Cytotoxicity against human sensitive MDA-MB-231 cells assessed as inhibition of cell growth after 72 hrs by Alamar Blue assay	Homo sapiens	2	ALA3600342	cell-based format	Scientific Literature
4.	ALA3602886	A	Cytotoxicity against human insensitive HCT116 cells assessed as inhibition of cell growth after 72 hrs by Alamar Blue assay	Homo sapiens	2	ALA3600342	cell-based format	Scientific Literature
5.	ALA3602887	B	Antagonist activity at full-length FLAG-tagged XIAP (unknown origin) transfected in HEK293 cells assessed as inhibition of interaction with caspase 9 after 2 hrs by immunoprecipitation assay	Homo sapiens	2	ALA3600342	cell-based format	Scientific Literature
6.	ALA3602888	B	Antagonist activity at cIAP1 in human EVSA-T cells after 2 hrs	Homo sapiens	1	ALA3600342	cell-based format	Scientific Literature
7.	ALA3602889	B	Antagonist activity at cIAP1 in human EVSA-T cells assessed as increase in cleaved caspase-3 level after 6 hrs	Homo sapiens	1	ALA3600342	cell-based format	Scientific Literature
8.	ALA3602890	F	Reduction of cIAP1 level in human MDA-MB-231 cells xenografted in Balb/c SCID mouse at 100 mg/kg, ip measured after 1 to 6 hrs by Western blot analysis relative to vehicle-treated control	Mus musculus	1	ALA3600342	organism-based format	Scientific Literature
9.	ALA3602891	F	Reduction of cIAP1 level in human MDA-MB-231 cells xenografted in Balb/c SCID mouse at 100 mg/kg, ip measured after 1 hr by MSD plate-based method relative to vehicle-treated control	Mus musculus	1	ALA3600342	organism-based format	Scientific Literature
10.	ALA3602892	F	Reduction of cIAP1 level in human MDA-MB-231 cells xenografted in Balb/c SCID mouse at 100 mg/kg, ip measured after 24 hrs by Western blot analysis	Mus musculus	1	ALA3600342	organism-based format	Scientific Literature
11.	ALA3602893	F	Reduction of XIAP level in human MDA-MB-231 cells xenografted in Balb/c SCID mouse at 100 mg/kg, ip measured after 1 to 24 hrs by Western blot analysis	Mus musculus	1	ALA3600342	organism-based format	Scientific Literature
12.	ALA3602894	F	Increase in cleaved PARP level in human MDA-MB-231 cells xenografted in Balb/c SCID mouse at 100 mg/kg, ip measured after 1 to 24 hrs by Western blot analysis	Mus musculus	1	ALA3600342	organism-based format	Scientific Literature
13.	ALA3602895	F	Increase in cleaved caspase-3 level in human MDA-MB-231 cells xenografted in Balb/c SCID mouse at 100 mg/kg, ip measured after 1 to 24 hrs by Western blot analysis	Mus musculus	1	ALA3600342	organism-based format	Scientific Literature
14.	ALA3602896	B	Antagonist activity at cIAP1-BIR3 domain (unknown origin) assessed as inhibition of interaction with SMAC by fluorescence polarization assay	Homo sapiens	8	ALA3600342	single protein format	Scientific Literature
15.	ALA3602963	F	Antitumor activity against human MDA-MB-231 cells xenografted in Balb/c SCID mouse assessed as tumor growth inhibition at 100 mg/kg, po bid measured every 3 days	Homo sapiens	1	ALA3600342	organism-based format	Scientific Literature
16.	ALA3602964	T	Toxicity in Balb/c SCID mouse xenografted with human MDA-MB-231 cells assessed as effect on body weight at 100 mg/kg, po bid measured everyday during compound dosing	Mus musculus	1	ALA3600342	organism-based format	Scientific Literature
17.	ALA3705917	B	In vitro Competitive Displacement Binding Assays : Modified SMAC peptides and compounds were tested for their ability to displace the fluorescent tracer from either XIAP, clAP-1 or clAP-2. BIR3 domains of clAP-1 , clAP-2 and XIAP were incubated with test compounds or SMAC based peptides and their respective peptide probes (Peptide Protein Research) in assay buffer (50mM Hepes pH 7.5, 0.025% Tween-20, 0.01 % BSA, and 1 mM DTT). Positive controls consisted of BIR3 proteins and tracer (no inhibition) and negative controls contained tracer only (100% inhibition). The samples were incubated at room temperature for 1 hr (XIAP and clAP-2) or 3hrs (clAP-1 ) prior to being read in the BMG Pherastar in Fluorescence Polarization mode (FP 485nm, 520nm, 520nm).		208	ALA3639218	single protein format	BindingDB Database
18.	ALA3707783	B	In vitro Competitive Displacement Binding Assays : Modified SMAC peptides and compounds were tested for their ability to displace the fluorescent tracer from either XIAP, clAP-1 or clAP-2. BIR3 domains of clAP-1 , clAP-2 and XIAP were incubated with test compounds or SMAC based peptides and their respective peptide probes (Peptide Protein Research) in assay buffer (50mM Hepes pH 7.5, 0.025% Tween-20, 0.01 % BSA, and 1 mM DTT). Positive controls consisted of BIR3 proteins and tracer (no inhibition) and negative controls contained tracer only (100% inhibition). The samples were incubated at room temperature for 1 hr (XIAP and clAP-2) or 3hrs (clAP-1 ) prior to being read in the BMG Pherastar in Fluorescence Polarization mode (FP 485nm, 520nm, 520nm).		162	ALA3639218	single protein format	BindingDB Database