# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3602821 | Binding | Binding affinity to purified recombinant full-length Aurora A (unknown origin) assessed as covalent irreversible complex formation at 2.4 uM irradiated for 15 mins with near-UV light by SDS-PAGE and Western blot method | Homo sapiens | 10 | ALA3600360 | single protein format | Scientific Literature | |
2. | ALA3602981 | Binding | Binding affinity to purified recombinant full-length Aurora A (unknown origin) by fluorescence polarisation/anisotropy based equilibrium binding assay | Homo sapiens | 13 | ALA3600360 | single protein format | Scientific Literature | |
3. | ALA3602982 | Binding | Inhibition of purified recombinant full-length Aurora A (unknown origin) assessed as residual enzyme activity at 2 mM using kemptide analogue substrate and 5 mM ATP in presence of TPX2 (1 to 43 residues) | Homo sapiens | 13 | ALA3600360 | single protein format | Scientific Literature | |
4. | ALA3813578 | Binding | Inhibition of Aurora A (unknown origin) | Homo sapiens | 1 | ALA3808344 | single protein format | Scientific Literature | |
5. | ALA3862723 | Binding | Inhibition of His-tagged human Aurora A kinase (122 to 40 residues) expressed in Escherichia coli BL21 (DE3) Rosetta cells using biotinylated STK2 substrate incubated for 30 mins by HTRF assay | Homo sapiens | 50 | ALA3861974 | cell-based format | Scientific Literature | |
6. | ALA3862725 | Binding | Inhibition of Aurora-B/INCENP (unknown origin) using biotinylated STK2 substrate incubated for 30 mins by HTRF assay | Homo sapiens | 13 | ALA3861974 | protein complex format | Scientific Literature | |
7. | ALA3862742 | Binding | Inhibition of Aurora A kinase autophosphorylation at Thr288 in human HeLa Kyoto cells incubated for 20 hrs | Homo sapiens | 4 | ALA3861974 | cell-based format | Scientific Literature | |
8. | ALA3862744 | Binding | Inhibition of Aurora B kinase in human HeLa Kyoto cells assessed as effect on distribution of phspho-histone H3 ser10 level incubated for 20 hrs | Homo sapiens | 4 | ALA3861974 | cell-based format | Scientific Literature | |
9. | ALA3871598 | Binding | Inhibition of human recombinant His-tagged Aurora A expressed in Escherichia coli using RRR(GLRRASLG)4R-NH2 as substrate after 40 mins in presence of [gamma-33P]-ATP by liquid scintillation counting | Homo sapiens | 1 | ALA3871274 | assay format | Scientific Literature | |
10. | ALA3871599 | Binding | Inhibition of human recombinant full-length N-terminal GST-tagged Aurora B (1 to 344 end residues) expressed in baculovirus expression system coexpressing His-tagged INCENP (803 to 918 end residues) using GLRRASLG-NH2 as substrate after 20 mins in presence of [gamma-33P]-ATP by liquid scintillation counting | Homo sapiens | 1 | ALA3871274 | assay format | Scientific Literature | |
11. | ALA3871600 | Binding | Inhibition of human recombinant full-length N-terminal GST-tagged Aurora C (1 to 275 end residues) expressed in baculovirus expression system using GLRRASLG-NH2 as substrate after 20 mins in presence of [gamma-33P]-ATP by liquid scintillation counting | Homo sapiens | 1 | ALA3871274 | assay format | Scientific Literature | |
12. | ALA3882306 | Binding | Inhibition of human N-terminal His-tagged Aurora A expressed in Escherichia coli BL21-Codon-Plus(DE3)-RIL cells using 5-carboxy-fluorescein-y-aminobutyric acid-Ala-Leu-Arg-Arg-Ala-Ser-Leu-Gly-NH2 as substrate after 120 mins by fluorescence polarization assay | Homo sapiens | 37 | ALA3882245 | cell-based format | Patent Bioactivity Data | |
13. | ALA3882307 | Binding | Inhibition of human full-length N-terminal GST-tagged Aurora B/His-tagged INCENP (803 to 916 residues) using kemptide as substrate and [gamma33P]ATP after 45 mins by liquid scintillation counting method | Homo sapiens | 37 | ALA3882245 | protein complex format | Patent Bioactivity Data | |
14. | ALA3882308 | Functional | Cytotoxicity against human HeLaS3 cells assessed as cell growth inhibition after 3 days by WST-8 assay | Homo sapiens | 21 | ALA3882245 | cell-based format | Patent Bioactivity Data | |
15. | ALA3882310 | Functional | Cytotoxicity against human HeLaS3 cells assessed as cell growth inhibition at 0.3 uM after 3 days by WST-8 assay | Homo sapiens | 18 | ALA3882245 | cell-based format | Patent Bioactivity Data | |
16. | ALA3882311 | Functional | Cytotoxicity against human HeLaS3 cells assessed as cell growth inhibition at 1 uM after 3 days by WST-8 assay | Homo sapiens | 11 | ALA3882245 | cell-based format | Patent Bioactivity Data | |
17. | ALA3882312 | Functional | Cytotoxicity in human HeLaS3 cells assessed as cell growth inhibition at 0.3 uM after 3 days in presence of docetaxel by WST-8 assay | Homo sapiens | 18 | ALA3882245 | cell-based format | Patent Bioactivity Data | |
18. | ALA3882313 | Functional | Cytotoxicity in human HeLaS3 cells assessed as cell growth inhibition at 1 uM after 3 days in presence of docetaxel by WST-8 assay | Homo sapiens | 11 | ALA3882245 | cell-based format | Patent Bioactivity Data | |
19. | ALA4266304 | Functional | Antiproliferation activity against human HCC1143 cells assessed as inhibition of cell proliferation | Homo sapiens | 1 | ALA4265875 | cell-based format | Scientific Literature | |
20. | ALA4266305 | Functional | Antiproliferation activity against human MCF7 cells assessed as inhibition of cell proliferation | Homo sapiens | 1 | ALA4265875 | cell-based format | Scientific Literature |