#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3619085	F	Antagonist activity at human glucagon receptor expressed in CHOK1 cells assessed as inhibition of glucagon-stimulated intracellular cAMP formation preincubated for 20 mins followed by glucagon stimulation measured after 30 mins by fluorescent plate reader analysis	Homo sapiens	6	ALA3616453	cell-based format	Scientific Literature
2.	ALA3619086	B	Displacement of [125I]-glucagon from human glucagon receptor expressed in Chem-1 cell membranes after 6 to 10 hrs by scintillation proximity assay	Homo sapiens	6	ALA3616453	cell-based format	Scientific Literature
3.	ALA3705610	B	SPA Assay: The Glucagon SPA assay is used to determine the ability of test compounds to block the binding of glucagon-cex to the glucagon receptor. Test compounds are re-suspended and serially diluted in 100% DMSO. 1 ul of test compound at the desired concentrations is spotted into the appropriate wells of 96 well low binding white clear bottom plate (Corning). 1 ul of DMSO is spotted into total binding wells. 1 ul of a known glucagon antagonist at a concentration of 20 uM is added to non specific binding wells. 0.3-0.75 ug of membrane from chem-1 cells stably transfected with the human glucagon receptor (Millipore), 125 pM of [125I]Glucagon-Cex (Perkin Elmer) and 175 ug of WGA PVT SPA beads (Perkin Elmer) are added to all wells of the assay plate. All assay ingredients with the exception of test compounds are re-suspended in the following buffer; 50 mM Hepes pH 7.4; 5 mM MgCl2; 1 mM CaCl; 5% glycerol and 0.2% BSA.	Homo sapiens	23	ALA3638779	assay format	BindingDB Database