| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3619967 | B | Inhibition of kelch domain of Keap1 (unknown origin) interaction to high affinity ETGE motif from Nrf2 unknown origin) at 100 uM by fluorescence polarization assay | Homo sapiens | 58 | protein format | Scientific Literature | ||
| 2. | ALA3619968 | B | Activation of Nrf2 in mouse Hepa1c1c7 cells assessed as induction of NQO1 production at 10 uM after 24 hrs by MTT colorimetric assay relative to control | Mus musculus | 61 | cell-based format | Scientific Literature | ||
| 3. | ALA3620075 | B | Inhibition of kelch domain of Keap1 (unknown origin) interaction to high affinity ETGE motif from Nrf2 by fluorescence polarization assay | Homo sapiens | 14 | protein format | Scientific Literature | ||
| 4. | ALA3620076 | B | Activation of Nrf2 in mouse Hepa1c1c7 cells assessed as concentration required to 2 fold induction of NQO1 production after 24 hrs by MTT colorimetric assay | Mus musculus | 11 | cell-based format | Scientific Literature | ||
| 5. | ALA3620078 | F | Increase of Nrf2 protein level in mouse Hepa1c1c7 cells at 10 uM after 24 hrs by Western blot analysis | Mus musculus | 4 | cell-based format | Scientific Literature | ||
| 6. | ALA3620080 | F | Increase of Nrf2 protein level in mouse Hepa1c1c7 cells at 10 uM by Western blot analysis | Mus musculus | 3 | cell-based format | Scientific Literature | ||
| 7. | ALA3620081 | F | Increase of Nrf2 protein level in mouse Hepa1c1c7 cells at 10 uM after 3 to 6 hrs by Western blot analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA3620083 | B | Activation of Nrf2 in mouse Hepa1c1c7 cells assessed as increase of HO-1 expression at 10 uM after 6 hrs by Western blot analysis | Mus musculus | 3 | cell-based format | Scientific Literature | ||
| 9. | ALA3620084 | B | Activation of Nrf2 in mouse Hepa1c1c7 cells assessed as increase of NQO1 expression at 10 uM measured before 24 hrs by Western blot analysis | Mus musculus | 3 | cell-based format | Scientific Literature | ||
| 10. | ALA3620085 | B | Inhibition of mCherry labeled Keap1 interaction to DLG motif from Nrf2 in human HEK293 cells at 10 uM by FRET based assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 11. | ALA3620086 | B | Inhibition of mCherry labeled Keap1 interaction to ETGE motif from Nrf2 in human HEK293 cells at 10 uM by FRET based assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 12. | ALA3620087 | A | Cytotoxicity against mouse Hepa1c1c7 cells assessed as cell viability at 200 uM after 24 to 48 hrs by SRB assay | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 13. | ALA3620089 | A | Therapeutic index, ratio of GI50 for mouse Hepa1c1c7 cells to CD for activation of Nrf2 in mouse Hepa1c1c7 cells assessed as concentration required to 2 fold induction of NQO1 production | Mus musculus | 2 | cell-based format | Scientific Literature | ||
| 14. | ALA3620090 | B | Inhibition of kelch domain of Keap1 (unknown origin) interaction to high affinity ETGE motif from Nrf2 unknown origin) in presence of 0 to 1 % v/v Triton X100 by fluorescence polarization assay | Homo sapiens | 1 | protein format | Scientific Literature | ||
| 15. | ALA3620091 | B | Binding affinity to kelch domain of Keap1 (unknown origin) using high affinity ETGE motif from Nrf2 unknown origin) at 200 uM by fluorescence polarization assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 16. | ALA4376060 | B | Binding affinity to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) assessed as thermal stabilization by sypro orange dye based thermal shift assay | Homo sapiens | 19 | assay format | Scientific Literature | ||
| 17. | ALA4376062 | B | Binding affinity to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) by surface plasmon resonance analysis | Homo sapiens | 21 | assay format | Scientific Literature | ||
| 18. | ALA4376066 | B | Binding affinity to Keap1 in mouse Hepa1c1c7 cells assessed as reduction in Keap1 interaction by measuring induction of NQO1 activity measured after 24 hrs | Mus musculus | 9 | cell-based format | Scientific Literature | ||
| 19. | ALA4376067 | B | Inhibition of Cys5-LDEETGEFL-NH2 binding to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) measured after 10 to 15 mins by fluorescence polarization assay | Homo sapiens | 25 | assay format | Scientific Literature | ||
| 20. | ALA4376068 | P | Solubility of the compound in 4 to 8% DMSO | 4 | small-molecule physicochemical format | Scientific Literature |
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