# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3632013 | B | Inhibition of Stat3 dimer DNA binding activity in mouse NIH3T3/vSrc nuclear extract at 5 uM preincubated for 30 mins followed by addition of radiolabeled probe hSIE for 30 mins by EMSA | Mus musculus | 24 | ALA3627612 | single protein format | Scientific Literature | |
2. | ALA3632014 | B | Inhibition of Stat1:Stat3 dimer DNA binding activity in EGF-stimulated mouse NIH3T3 nuclear extract expressing human EGFR assessed as residual activity at 5 uM preincubated for 30 mins followed by addition of radiolabeled probe hSIE for 30 mins by EMSA | Mus musculus | 6 | ALA3627612 | cell-based format | Scientific Literature | |
3. | ALA3632015 | B | Inhibition of Stat3 dimer DNA binding activity in EGF-stimulated mouse NIH3T3 nuclear extract expressing human EGFR assessed as residual activity at 5 uM preincubated for 30 mins followed by addition of radiolabeled probe hSIE for 30 mins by EMSA | Mus musculus | 6 | ALA3627612 | single protein format | Scientific Literature | |
4. | ALA3632016 | B | Inhibition of Stat1 dimer DNA binding activity in EGF-stimulated mouse NIH3T3 nuclear extract expressing human EGFR assessed as residual activity at 5 uM preincubated for 30 mins followed by addition of radiolabeled probe hSIE for 30 mins by EMSA | Mus musculus | 6 | ALA3627612 | single protein format | Scientific Literature | |
5. | ALA3632017 | B | Inhibition of Stat5 dimer DNA binding activity in EGF-stimulated mouse NIH3T3 nuclear extract expressing human EGFR at 5 uM preincubated for 30 mins followed by addition of radiolabeled probe MGFe for 30 mins by EMSA | Mus musculus | 11 | ALA3627612 | cell-based format | Scientific Literature | |
6. | ALA3632018 | B | Inhibition of Stat1 dimer DNA binding activity in EGF-stimulated mouse NIH3T3 nuclear extract expressing human EGFR at 5 uM preincubated for 30 mins followed by addition of radiolabeled probe hSIE for 30 mins by EMSA | Mus musculus | 12 | ALA3627612 | single protein format | Scientific Literature | |
7. | ALA3632022 | B | Inhibition of Stat3 activity in human U251MG cells assessed as phosphorylated Stat3 level at 5 uM by immunoblot analysis | Homo sapiens | 5 | ALA3627612 | cell-based format | Scientific Literature | |
8. | ALA3632141 | B | Modulation of EGF-induced Stat1 phosphorylation in mouse NIH3T3 cells expressing human EGFR at 5 uM preincubated for 3 hrs followed by stimulation with 1 ug/ml EGF for 12 mins by immunoblot analysis | Mus musculus | 4 | ALA3627612 | cell-based format | Scientific Literature | |
9. | ALA3632142 | B | Modulation of EGF-induced Stat5 phosphorylation in mouse NIH3T3 cells expressing human EGFR at 5 uM preincubated for 3 hrs followed by stimulation with 1 ug/ml EGF for 12 mins by immunoblot analysis | Mus musculus | 4 | ALA3627612 | cell-based format | Scientific Literature | |
10. | ALA3632143 | B | Modulation of EGF-induced Erk1/2 phosphorylation in mouse NIH3T3 cells expressing human EGFR at 5 uM preincubated for 3 hrs followed by stimulation with 1 ug/ml EGF for 12 mins by immunoblot analysis | Mus musculus | 4 | ALA3627612 | cell-based format | Scientific Literature | |
11. | ALA3632144 | B | Inhibition of EGF-induced Stat3 phosphorylation in mouse NIH3T3 cells expressing human EGFR at 5 uM preincubated for 3 hrs followed by stimulation with 1 ug/ml EGF for 12 mins by immunoblot analysis | Mus musculus | 4 | ALA3627612 | cell-based format | Scientific Literature | |
12. | ALA3632145 | B | Modulation of Akt phosphorylation in human U251MG cells at 5 uM after 0.5 to 24 hrs by immunoblot analysis | Homo sapiens | 4 | ALA3627612 | cell-based format | Scientific Literature | |
13. | ALA3632146 | B | Modulation of Erk1/2 phosphorylation in human U251MG cells at 5 uM after 0.5 to 24 hrs by immunoblot analysis | Homo sapiens | 4 | ALA3627612 | cell-based format | Scientific Literature | |
14. | ALA3632147 | F | Effect on SOCS3 expression in human U251MG cells at 5 uM after 0.5 to 24 hrs by immunoblot analysis | Homo sapiens | 4 | ALA3627612 | cell-based format | Scientific Literature | |
15. | ALA3632148 | F | Effect on PTP1B expression in human U251MG cells at 5 uM after 0.5 to 24 hrs by immunoblot analysis | Homo sapiens | 4 | ALA3627612 | cell-based format | Scientific Literature | |
16. | ALA3632149 | B | Inhibition of orthovandate-induced Stat3 phosphorylation in human U251MG cells at 5 uM for 3 hrs by immunoblot analysis in presence of 10 to 300 uM orthovandate | Homo sapiens | 4 | ALA3627612 | cell-based format | Scientific Literature | |
17. | ALA3632157 | F | Antiproliferative activity against human U251MG cells for 72 hrs by cyquant assay | Homo sapiens | 9 | ALA3627612 | cell-based format | Scientific Literature | |
18. | ALA3632158 | F | Antiproliferative activity against human MDA-MB-231 cells for 72 hrs by cyquant assay | Homo sapiens | 5 | ALA3627612 | cell-based format | Scientific Literature | |
19. | ALA3632159 | F | Antiproliferative activity against human U373MG cells for 72 hrs by cyquant assay | Homo sapiens | 5 | ALA3627612 | cell-based format | Scientific Literature | |
20. | ALA3632160 | F | Antiproliferative activity against human SF295 cells for 72 hrs by cyquant assay | Homo sapiens | 5 | ALA3627612 | cell-based format | Scientific Literature |