# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3632013 | B | Inhibition of Stat3 dimer DNA binding activity in mouse NIH3T3/vSrc nuclear extract at 5 uM preincubated for 30 mins followed by addition of radiolabeled probe hSIE for 30 mins by EMSA | Mus musculus | 24 | single protein format | Scientific Literature | ||
2. | ALA3632018 | B | Inhibition of Stat1 dimer DNA binding activity in EGF-stimulated mouse NIH3T3 nuclear extract expressing human EGFR at 5 uM preincubated for 30 mins followed by addition of radiolabeled probe hSIE for 30 mins by EMSA | Mus musculus | 12 | single protein format | Scientific Literature | ||
3. | ALA3632020 | B | Inhibition of Stat3 dimer DNA binding activity in human U251MG cells nuclear extract after 1.5 hrs by EMSA using radiolabeled probe hSIE | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
4. | ALA3632023 | B | Inhibition of Stat3 activity in human U251MG cells assessed as phosphorylated Stat3 level after 2 hrs by immunoblot analysis | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
5. | ALA3632029 | B | Inhibition of Stat1:Stat3 dimer DNA binding activity in EGF-stimulated mouse NIH3T3 nuclear extract expressing human EGFR at 5 uM preincubated for 30 mins followed by addition of radiolabeled probe hSIE for 30 mins by EMSA | Mus musculus | 1 | cell-based format | Scientific Literature | ||
6. | ALA3632139 | B | Inhibition of Stat3 dimer DNA binding activity in EGF-stimulated mouse NIH3T3 nuclear extract expressing human EGFR at 5 uM preincubated for 30 mins followed by addition of radiolabeled probe hSIE for 30 mins by EMSA | Mus musculus | 1 | single protein format | Scientific Literature | ||
7. | ALA3632140 | B | Inhibition of Stat3 activity in human U251MG cells assessed as phosphorylated Stat3 level at 5 uM after 6 to 24 hrs by immunoblot analysis | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
8. | ALA3632157 | F | Antiproliferative activity against human U251MG cells for 72 hrs by cyquant assay | Homo sapiens | 9 | cell-based format | Scientific Literature | ||
9. | ALA3630073 | A | Cytotoxicity against mouse NIH/3T3 cells after 72 hrs by cyquant cells | Mus musculus | 9 | cell-based format | Scientific Literature | ||
10. | ALA3630077 | F | Antiproliferative activity against human U251MG cells at 5 uM for 72 hrs by cyquant assay | Homo sapiens | 25 | cell-based format | Scientific Literature |