#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3630319	B	Inhibition of Mycobacterium smegmatis DNA gyrase B expressed in Escherichia coli BL21 (DE3) pLysS cells assessed as reduction in ATPase activity incubated for 100 mins by inorganic phosphate detection assay	Mycolicibacterium smegmatis	50		cell-based format	Scientific Literature
2.	ALA3630320	B	Inhibition of Staphylococcus aureus DNA gyrase B assessed as reduction in ATPase activity at 100 uM incubated for 100 mins by inorganic phosphate detection assay	Staphylococcus aureus	1		single protein format	Scientific Literature
3.	ALA3630321	B	Inhibition of Escherichia coli Inspiralis DNA gyrase B assessed as reduction in ATPase activity at 100 uM incubated for 100 mins by inorganic phosphate detection assay	Escherichia coli	1		single protein format	Scientific Literature
4.	ALA3630322	B	Inhibition of DNA gyrase GyrA/GyrB in Mycobacterium tuberculosis incubated for 30 mins assessed as reduction in supercoiling of relaxed relaxed pBR322 DNA by ethidium bromide staining based agarose gel electrophoresis method	Mycobacterium tuberculosis	50		assay format	Scientific Literature
5.	ALA3630438	F	Antimycobacterial activity against Mycobacterium tuberculosis H37Rv assessed as growth inhibition incubated for 7 days by microplate alamar blue assay	Mycobacterium tuberculosis	50		organism-based format	Scientific Literature
6.	ALA3630439	A	Cytotoxicity against mouse RAW264.7 cells assessed as inhibition of cell viability at 50 uM incubated for 48 hrs at 37 degC by MTT assay relative to control	Mus musculus	50		cell-based format	Scientific Literature
7.	ALA3630440	B	Binding affinity to Mycobacterium smegmatis DNA gyrase B assessed as changing in melting temperature of the protein-ligand complex by differential scanning fluorometry	Mycolicibacterium smegmatis	1		single protein format	Scientific Literature