| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3637037 | B | Inhibition of Keap1-NRF2 interaction (unknown origin) expressed in Escherichia coli BL21 (DE3) cells assessed as reduction in FITC-9mer NRF2 peptide binding after 1 hr by fluorescence anisotropy assay | Homo sapiens | 33 | cell-based format | Scientific Literature | ||
| 2. | ALA3637038 | B | Binding affinity to the Keap1 (321 to 609 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells by surface plasmon resonance assay | Homo sapiens | 25 | cell-based format | Scientific Literature | ||
| 3. | ALA4152127 | P | Kinetic solubility in pH 7.4 incubated for 1 hr by HPLC method | 18 | small-molecule physicochemical format | Scientific Literature | |||
| 4. | ALA4152128 | B | Inhibition of Keap1 Kelch domain (unknown origin) /Nrf2 (unknown origin) interaction using fluorescein-labeled 9-mer peptide containing NRF2 Neh2 domain ETGE motif by fluorescence anisotropy assay | Homo sapiens | 18 | protein format | Scientific Literature | ||
| 5. | ALA4152129 | B | Binding affinity to Keap1 Kelch domain (unknown origin) by surface plasmon resonance assay | Homo sapiens | 18 | single protein format | Scientific Literature | ||
| 6. | ALA4152130 | B | Inhibition of Keap1/Nrf2 interaction in human HaCaT cells assessed as increase in NRF2 protein expression at 10 uM incubated for 6 hrs by immunoblot assay | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
| 7. | ALA4152131 | B | Inhibition of Keap1/Nrf2 interaction in human HaCaT cells assessed as increase in NQO1 protein expression at 10 uM incubated for 6 hrs by immunoblot assay | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
| 8. | ALA4152132 | B | Inhibition of Keap1/Nrf2 interaction in human HaCaT cells assessed as increase in NQO1 protein expression at 10 uM incubated for 24 hrs by immunoblot assay | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
| 9. | ALA4152133 | B | Inhibition of Keap1/Nrf2 interaction in human HaCaT cells assessed as increase in NRF2 protein expression at 10 uM incubated for 24 hrs by immunoblot assay | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
| 10. | ALA4152134 | B | Inhibition of Keap1/Nrf2 interaction in human HaCaT cells assessed as increase in GCLM protein expression at 10 uM incubated for 24 hrs by immunoblot assay | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
| 11. | ALA4152149 | A | Stability in human liver microsomes assessed as compound remaining level at 200 uM incubated for 20 mins by LC-MS method | Homo sapiens | 6 | microsome format | Scientific Literature | ||
| 12. | ALA4152150 | A | Stability in mouse liver microsomes assessed as compound remaining level at 200 uM incubated for 20 mins by LC-MS method | Mus musculus | 6 | microsome format | Scientific Literature | ||
| 13. | ALA4376060 | B | Binding affinity to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) assessed as thermal stabilization by sypro orange dye based thermal shift assay | Homo sapiens | 19 | assay format | Scientific Literature | ||
| 14. | ALA4376062 | B | Binding affinity to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) by surface plasmon resonance analysis | Homo sapiens | 21 | assay format | Scientific Literature | ||
| 15. | ALA4376065 | B | Binding affinity to Keap1 in mouse Hepa1c1c7 cells assessed as reduction in Keap1 interaction by measuring induction of NQO1 activity at 1 to 10 uM measured after 24 hrs | Mus musculus | 10 | cell-based format | Scientific Literature | ||
| 16. | ALA4376067 | B | Inhibition of Cys5-LDEETGEFL-NH2 binding to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) measured after 10 to 15 mins by fluorescence polarization assay | Homo sapiens | 25 | assay format | Scientific Literature | ||
| 17. | ALA4376091 | U | Redox cycling activity of the compound assessed as H2O2 production measured after 15 mins in presence of TCEP by phenol red reagent based horseradish peroxidase coupled assay | 21 | assay format | Scientific Literature | |||
| 18. | ALA4376092 | B | Ratio of IC50 for inhibition of Cys5-LDEETGEFL-NH2 binding to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) in absence of 0.01% Triton X-100 to IC50 for inhibition of Cys5-LDEETGEFL-NH2 binding to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) in presence of 0.01% Triton X-100 | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 19. | ALA4376101 | B | Binding affinity to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) assessed as change in melting temperature by sypro orange dye based thermal shift assay | Homo sapiens | 11 | assay format | Scientific Literature | ||
| 20. | ALA5230556 | B | Binding affinity to human KEAP1 by SPR direct binding assay | Homo sapiens | 7 | single protein format | Scientific Literature |
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