Inhibition of TPO (unknown origin) using Amplex Red as substrate assessed as formation of resorufin by measuring ratio of Kinact to Ki measured every 20 secs by spectrophotometric analysis
Inhibition of peroxidase activity of MPO isolated from human polynuclear leukocytes using Amplex Red as substrate assessed as partition ratio preincubated for 15 mins with H2O2 followed by 300 fold dilution measured immediately post dilution by spectrophotometric analysis
Inhibition of peroxidase activity of MPO isolated from human polynuclear leukocytes using Amplex Red as substrate assessed as formation of resorufin measured every 20 secs by spectrophotometric analysis
Inhibition of peroxidase activity of MPO isolated from human polynuclear leukocytes using Amplex Red as substrate assessed as formation of resorufin by measuring ratio of Kinact to Ki measured every 20 secs by spectrophotometric analysis
Drug metabolism in sodium phosphate buffer assessed as formation of GSH adduct at 10 uM after 60 mins by LC-MS/MS analysis in presence of human MPO/H2O2/Cl-/excess GSH