Antitumor activity against human HCT116 cells xenografted in athymic nude BALB/cAJcL mouse assessed as inhibition of tumor growth at 75 mg/kg, iv administered on days 1,3,5,8,10 and 12 measured on day 15
Antitumor activity against human HCT116 cells xenografted in iv dosed athymic nude BALB/cAJcL mouse assessed as inhibition of tumor growth drug administered on days 1,3,5,8,10 and 12 measured on day 15
Antitumor activity against human HCT116 cells xenografted in athymic nude BALB/cAJcL mouse assessed as inhibition of tumor growth at 25 mg/kg, iv administered on days 1,3,5,8,10 and 12 measured on day 15
Inhibition of His-tagged KSP motor domain (1 to 369) (unknown origin) assessed as inhibition of microtubule-stimulated KSP ATPase activity preincuabted for 30 mins followed by ATP addition measured after 15 mins by Kinase-Glo assay
Cell cycle arrest at mitotic phase in human HeLa cells assessed as formation of monopolar spindles at 40 nM after 6 hrs by DAPI staining-based microscopic analysis
Toxicity in athymic nude BALB/cAJcL mouse xenografted with HCT116 cells assessed as body weight loss at 25 mg/kg, iv administered on days 1,3,5,8,10 and 12 relative to vehicle-treated control
Inhibition C-terminal His-tagged KSP motor domain(1 to 369 residues) (unknown origin) expressed in bacteria assessed as inhibition of microtubule-stimulated ATPase activity preincubated for 30 mins followed by addition of ATP and measured after 15 mins by luciferase-derived luminiscence assay
Inhibition of recombinant human GST tagged Kinesin spindle protein assessed as inhibition of ATPase activity in presence of ATP at 2 uM measured by plate reader method