# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3636369 | B | Inhibition of TPO (unknown origin) using Amplex Red as substrate assessed as formation of resorufin by measuring ratio of Kinact to Ki measured every 20 secs by spectrophotometric analysis | Homo sapiens | 9 | ALA3632461 | single protein format | Scientific Literature | |
2. | ALA3636508 | P | Lipophilicity, log D of the compound at pH 7.4 by shake-flask method | 9 | ALA3632461 | small-molecule physicochemical format | Scientific Literature | ||
3. | ALA3636509 | B | Inhibition of peroxidase activity of MPO isolated from human polynuclear leukocytes using Amplex Red as substrate assessed as formation of resorufin measured every 20 secs by spectrophotometric analysis | Homo sapiens | 6 | ALA3632461 | assay format | Scientific Literature | |
4. | ALA3636510 | B | Inhibition of peroxidase activity of MPO isolated from human polynuclear leukocytes using Amplex Red as substrate assessed as formation of resorufin by measuring ratio of Kinact to Ki measured every 20 secs by spectrophotometric analysis | Homo sapiens | 9 | ALA3632461 | assay format | Scientific Literature | |
5. | ALA3636511 | B | Irreversible inhibition of MPO in LPS-stimulated human whole blood after 4 hrs by Amplex Red/H2O2-based fluorescence plate reader analysis | Homo sapiens | 9 | ALA3632461 | single protein format | Scientific Literature | |
6. | ALA3636516 | A | Drug metabolism assessed as formation of human MPO-mediated (S)-2-amino-5-((R)-1-(carboxymethylamino)-3-(6-(2,4-dimethoxyphenyl)-1-(2-isopropoxyethyl)-4-oxo-1,4-dihydropyrimidin-2-ylthio)-1-oxopropan-2-ylamino)-5-oxopentanoic acid at 10 uM after 60 mins by LC-MS/MS analysis in presence of H2O2/Cl-/excess GSH | Homo sapiens | 1 | ALA3632461 | single protein format | Scientific Literature | |
7. | ALA3636517 | P | Retention time of the compound by LC-MS/MS analysis in presence of human MPO/H2O2/Cl-/excess GSH | 2 | ALA3632461 | small-molecule physicochemical format | Scientific Literature | ||
8. | ALA3636521 | A | Apparent permeability across RRCK cells | Canis lupus familiaris | 7 | ALA3632461 | cell-based format | Scientific Literature | |
9. | ALA3636525 | A | Fraction unbound in human plasma by equilibrium dialysis method | Homo sapiens | 6 | ALA3632461 | cell-free format | Scientific Literature | |
10. | ALA3636526 | A | Intrinsic apparent clearance in human liver microsomes | Homo sapiens | 7 | ALA3632461 | assay format | Scientific Literature | |
11. | ALA3636527 | A | Intrinsic apparent clearance in human hepatocytes assessed per 10'6 cells | Homo sapiens | 7 | ALA3632461 | cell-based format | Scientific Literature |