Drug metabolism assessed as (2S,3R,10R,13R,17S)-2,3,6-trihydroxy-10,13-dimethyl-17-((2R,3R)-2,3,6-trihydroxy-6-methylheptan-2-yl)-3,4,10,11,12,13,16,17-octahydro-1H-cyclopenta[a]phenanthren-7(2H)-one formation at 80 degC after 4 days
Drug level in aqueous solution treated with (2S,3R,10R,13R,14S,17S)-2,3,6,14-tetrahydroxy-10,13-dimethyl-17-((2R,3R)-2,3,6-trihydroxy-6-methylheptan-2-yl)-3,4,10,11,12,13,14,15,16,17-decahydro-1H-cyclopenta[a]phenanthren-7(2H)-one at pH 8
Drug level in aqueous solution treated with 20-hydroxyecdysone at 120 mg incubated for 2 days measured at 0.5 to 48 hrs by HPLC-DAD analysis in presence of NaOH
Induction of Akt phosphorylation in mouse C2C12 cells at 30 ug/ml pretreated with compound for 24 hrs followed by fresh medium with compound addition measured after 2 hrs by Western blot analysis