#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3705422	B	Fluorescence Polarization Competition Immunoassay: An autophosphorylation, fluorescence polarization competition immunoassay was used to determine the potency for c-fms inhibition exhibited by selected compounds of Formula I. The assay was performed in black 96-well microplates (LJL BioSystems). The assay buffer used was 100 mM 4-(2-hydroxyethyl)piperazine 1-ethanesulfonic acid (HEPES), pH 7.5, 1 mM 1,4-dithio-DL-threitol (DTT), 0.01% (v/v) Tween-20. Compounds were diluted in assay buffer containing 4% dimethylsulfoxide (DMSO) just prior to the assay. To each well, 5 μL of compound were added followed by the addition of 3 μL of a mix containing 33 nM c-fms (Johnson & Johnson PRD) and 16.7 mM MgCl2 (Sigma) in assay buffer. The kinase reaction was initiated by adding 2 μL of 5 mM ATP (Sigma) in assay buffer. The final concentrations in the assay were 10 nM c-fms, 1 mM ATP, 5 mM MgCl2, 2% DMSO.	Mus musculus	2		single protein format	BindingDB Database
2.	ALA4256963	B	Inhibition of c-FMS auto-phosphorylation (unknown origin) preincubated with enzyme followed by ATP addition measured after 45 mins by PTK green tracer based fluorescence polarization assay	Homo sapiens	2		single protein format	Patent Bioactivity Data
3.	ALA4256964	B	Inhibition of c-FMS in mouse BMDM assessed as reduction in CSF-1 stimulated cell proliferation by measuring BrDU incorporation treated for 30 hrs followed by BrDU addition during last 6 hrs of incubation by ELISA	Mus musculus	2		single protein format	Patent Bioactivity Data