#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3706142	B	Radioligand Competition Binding Assay: The affinity of the test compounds was determined by radioligand competition binding assay, using the known compound [3H]Ro-15-1788 (Flumazenil) (Perkin Elmer, 85.4 Ci/mmol) and the human recombinant GABA A receptor containing the alpha2, beta2, and gamma3 subunits.Membranes were prepared from HEK cells expressing hGABA A alpha2beta2- gamma3 receptor, and validated to ascertain protein concentration, receptor expression and to determine the Kd of the flumazenil as well as the Ki of a standard set of compounds before being used to test new compounds.The assay was carried out in 96 well plates; testing compounds using a 10 point semi-log dilution range from 19 uM top concentration. 100 ul of radioligand and 100 ul of membrane in 50 mM Tris-HCI and 0.05% F127 with 1 ul of test compound was incubated for 2 hours to allow the reaction to achieve equilibrium, and then harvested onto filter plates, dried and counted on a TopCount NXT. The data was analysed, and the Ki values were presented.		50		cell-based format	BindingDB Database
2.	ALA4351503	B	Displacement of [3H]Ro-15-1788 from human GABA alpha2/beta2/gamma2 receptor expressed in HEK cell membranes measured after 2 hrs by topcount method	Homo sapiens	50		cell-based format	Scientific Literature
3.	ALA4351505	A	Positive allosteric modulation of human GABAA alpha1/beta3/gamma2 expressed in HEK293 cells assessed as increase in GABA-stimulated chloride current response at 100 times Ki measured after 15 secs by QPatch electrophysiology method relative to GABA	Homo sapiens	50		cell-based format	Scientific Literature
4.	ALA4351506	B	Positive allosteric modulation of human GABAA alpha2/beta2/gamma2 expressed in HEK293 cells assessed as increase in GABA-stimulated chloride current response at 100 times Ki measured after 15 secs by QPatch electrophysiology method relative to GABA	Homo sapiens	50		cell-based format	Scientific Literature
5.	ALA4351508	P	Lipophilicity, log D of the compound		34		small-molecule physicochemical format	Scientific Literature
6.	ALA4351509	A	Intrinsic clearance in human liver microsomes	Homo sapiens	46		microsome format	Scientific Literature
7.	ALA4351510	A	Efflux ratio of permeability across apical to basolateral side over basolateral to apical side in MDCK-MDR1 cells at 2 uM	Canis lupus familiaris	46		cell-based format	Scientific Literature