#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3706109	B	Fluorescence Polarisation Assay: In a black, 384-well polystyrene assay plate, 30 microliters/well of 5 nM Escherichia coli DNA gyrase A/B tetramer and 130 micrograms/ml of topologically relaxed plasmid containing the triplex-forming sequence TTCTTCTTCTTCTTCTTCTTCTTCTTC in an assay buffer consisting of 35 mM Tris-HCl (pH 7.5), 24 mM KCl, 4 mM MgCl2, 2 mM dithiothreitol, 1.8 mM spermidine, 5% (v/v) glycerol, 200 nM bovine serum albumin, 0.8% dimethylsulfoxide, and 0.3 mM ATP may be incubated at ambient temperature for (typically 30 minutes) in the absence or presence of 5-10 different concentrations of test compound. The supercoiling reactions may be quenched by the addition of 10 microliters/well of 40 nM oligodeoxynucleotide probe in 3x triplex-forming buffer consisting of 150 mM NaCl, and 150 mM sodium acetate at pH 3.5. The oligodeoxynucleotide probe may be 5x-BODIPY-FL-labeled TTCTTCTTC. After 60 minutes, the fluorescence anisotropy of the BODIPY-FL may be measured in a Tecan Ultra plate reader, using 485 nM.	Escherichia coli	190	ALA3638965	protein complex format	BindingDB Database
2.	ALA4730280	B	Inhibition of recombinant Escherichia coli DNA gyrase AB expressed in Escherichia coli BL21 (DE3) using relaxed pJT519 DNA as substrate preincubated for 20 mins followed by 5'-BODIPY-TMR-TTCTTCTTCT addition and measured after 1 hr by fluorescence anisotropy	Escherichia coli	34	ALA4725408	assay format	Scientific Literature
3.	ALA4730281	P	n-octanol/phosphate buffer partition coefficient, log D of the compound at pH 7.4 measured after 1 hr by UV-LC/MS based shake flask method		34	ALA4725408	small-molecule physicochemical format	Scientific Literature
4.	ALA4730282	P	Solubility of the compound by high throughput solubility assay		34	ALA4725408	small-molecule physicochemical format	Scientific Literature
5.	ALA4730283	A	Protein binding in human plasma assessed as unbound fraction at 10 uM measured after 16 hrs by LC-MS/MS based equilibrium dialysis method	Homo sapiens	34	ALA4725408	cell-free format	Scientific Literature
6.	ALA4730284	F	Antibacterial activity against methicillin sensitive Staphylococcus aureus ARC516 by broth microdilution method	Staphylococcus aureus	34	ALA4725408	organism-based format	Scientific Literature
7.	ALA4730285	F	Antibacterial activity against methicillin sensitive Staphylococcus aureus ARC516 in presence of human serum by broth microdilution method	Staphylococcus aureus	34	ALA4725408	organism-based format	Scientific Literature
8.	ALA4730286	F	Antibacterial activity against methicillin/quinolone-resistant Staphylococcus aureus ARC517 by broth microdilution method	Staphylococcus aureus	34	ALA4725408	organism-based format	Scientific Literature
9.	ALA4730287	U	Ratio of MIC for antibacterial activity against methicillin/quinolone-resistant Staphylococcus aureus ARC517 to protein binding in human plasma assessed as unbound fraction		9	ALA4725408	assay format	Scientific Literature
10.	ALA4730288	F	Antibacterial activity against Escherichia coli ARC523 by broth microdilution method	Escherichia coli	34	ALA4725408	organism-based format	Scientific Literature
11.	ALA4730289	F	Antibacterial activity against Streptococcus pneumoniae ARC548 by broth microdilution method	Streptococcus pneumoniae	26	ALA4725408	organism-based format	Scientific Literature
12.	ALA4730290	F	Antibacterial activity against Streptococcus pyogenes ARC838 by broth microdilution method	Streptococcus pyogenes	26	ALA4725408	organism-based format	Scientific Literature
13.	ALA4730291	F	Antibacterial activity against Haemophilus influenzae ARC737 by broth microdilution method	Haemophilus influenzae	26	ALA4725408	organism-based format	Scientific Literature
14.	ALA4730292	A	Protein binding in mouse plasma assessed as unbound fraction at 10 uM measured after 16 hrs by LC-MS/MS based equilibrium dialysis method	Mus musculus	3	ALA4725408	cell-free format	Scientific Literature
15.	ALA4730293	A	Protein binding in rat plasma assessed as unbound fraction at 10 uM measured after 16 hrs by LC-MS/MS based equilibrium dialysis method	Rattus norvegicus	3	ALA4725408	cell-free format	Scientific Literature
16.	ALA4730294	A	Plasma clearance in CD-1 mouse at 3 mg/kg, iv administered as 15 mins infusion by LC-MS/MS analysis	Mus musculus	3	ALA4725408	organism-based format	Scientific Literature
17.	ALA4730295	A	Plasma clearance in Wistar Han rat at 3 mg/kg, iv administered as 15 mins infusion by LC-MS/MS analysis	Rattus norvegicus	10	ALA4725408	organism-based format	Scientific Literature
18.	ALA4730296	A	Unbound plasma clearance in CD-1 mouse at 3 mg/kg, iv administered as 15 mins infusion by LC-MS/MS analysis	Mus musculus	3	ALA4725408	organism-based format	Scientific Literature
19.	ALA4730297	A	Unbound plasma clearance in Wistar Han rat at 3 mg/kg, iv administered as 15 mins infusion by LC-MS/MS analysis	Rattus norvegicus	10	ALA4725408	organism-based format	Scientific Literature
20.	ALA4730299	A	Volume of distribution at steady state in Wistar Han rat at 3 mg/kg, iv administered as 15 mins infusion by LC-MS/MS analysis	Rattus norvegicus	3	ALA4725408	organism-based format	Scientific Literature