#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3705101	B	Activity Assay: The primary assay for compound inhibitory activity was the ATP depletion assay using the PDKtide substrate purchase from Millipore Corporation.	Homo sapiens	21		single protein format	BindingDB Database
2.	ALA3705102	B	Binding Assay: The secondary assay was a time resolved-FRET LanthaScreen Kinase Binding Assay. (Invitrogen Corporation, Carlsbad, Calif.). This assay evaluates the ability of the test compound to compete with a fluorescently-labeled tracer molecule to bind in the ATP pocket of a kinase.	Homo sapiens	19		single protein format	BindingDB Database
3.	ALA4220017	B	Inhibition of PDK1 mediated PI3K/Akt/mTOR pathway activation in human MV4-11 cells assessed as downregulation of DUSP4 mRNA expression level at 0.01 to 1 uM by RT-qPCR method	Homo sapiens	1		cell-based format	Scientific Literature
4.	ALA4220018	B	Inhibition of PDK1 (unknown origin) after 1 hr in presence of Ser/Thr-07 by fluorometric assay	Homo sapiens	26		single protein format	Scientific Literature
5.	ALA4220023	F	Antiproliferative activity against human AN3CA cells harboring PTEN/PIK3R1 double mutant after 96 hrs by ATP lite assay	Homo sapiens	2		cell-based format	Scientific Literature
6.	ALA4220024	F	Antiproliferative activity against human RL95-2 cells harboring PTEN/HRAS double mutant after 96 hrs by ATP lite assay	Homo sapiens	2		cell-based format	Scientific Literature
7.	ALA4220026	F	Antiproliferative activity against human Hs578T cells harboring PIK3R1 mutant in presence of PTEN shRNA after 96 hrs by ATP lite assay	Homo sapiens	2		cell-based format	Scientific Literature
8.	ALA4220028	B	Inhibition of PDK1 mediated PI3K/Akt/mTOR pathway activation in human AN3CA cells assessed as reduction in ribosomal p70S6 protein phosphorylation after 2 hrs by in-cell Western blotting analysis	Homo sapiens	2		cell-based format	Scientific Literature
9.	ALA4220030	F	Antiproliferative activity against human IOMM-Lee cells	Homo sapiens	2		cell-based format	Scientific Literature
10.	ALA4220031	F	Antiproliferative activity against human MDA-MB-231 cells	Homo sapiens	2		cell-based format	Scientific Literature
11.	ALA4220032	F	Antiproliferative activity against human KG1 cells	Homo sapiens	2		cell-based format	Scientific Literature
12.	ALA4220033	F	Antiproliferative activity against human MV4-11 cells	Homo sapiens	2		cell-based format	Scientific Literature
13.	ALA4220034	F	Antiproliferative activity against human KATO III cells	Homo sapiens	2		cell-based format	Scientific Literature
14.	ALA4220035	F	Antiproliferative activity against human Kasumi-1 cells	Homo sapiens	2		cell-based format	Scientific Literature
15.	ALA4220036	F	Antiproliferative activity against human AN3CA cells	Homo sapiens	2		cell-based format	Scientific Literature
16.	ALA4220037	F	Antiproliferative activity against human MFE296 cells	Homo sapiens	2		cell-based format	Scientific Literature
17.	ALA4220038	F	Antiproliferative activity against human DMS114 cells	Homo sapiens	2		cell-based format	Scientific Literature
18.	ALA4220040	F	Antiproliferative activity against human SK-ES-1 cells	Homo sapiens	2		cell-based format	Scientific Literature
19.	ALA4220041	F	Antiproliferative activity against HEL cells	Homo sapiens	2		cell-based format	Scientific Literature
20.	ALA4220042	F	Antiproliferative activity against human TC71 cells	Homo sapiens	2		cell-based format	Scientific Literature