#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3705617	B	Radioligand Binding Assay: Radioligand Binding Studies: Saturation experiments. A representative compound of formula (I) was tritiated. Three tritiums were incorporated in place of methyl hydrogens to generate [3H]compound. Binding of this radioligand was preformed in 5 mL borosilicate glass test tubes at room temperature. Binding was initiated by adding membranes to increasing concentrations of [3H]compound in 100 mM NaCl, 20 mM Tris HCl, pH 7.4 buffer containing 0.01% w/v bovine serum albumin (BSA) for 18 h. Non-specific binding was determined in the presence of 1 uM unlabelled compound. After 18 h, the reactants were filtered through GF/C glass fiber filters presoaked in 0.5% w/v polyethylene imine. Filters were washed with 15 mL ice cold 100 mM NaCl, 20 mM Tris HCl, pH7.4 buffer containing 0.25% BSA to separate bound from free ligand. [3H]compound bound to filters was quantified by liquid scintillation counting.Competitive binding experiments.		398	ALA3638545	assay format	BindingDB Database
2.	ALA3707743	B	Electrophysiological Assay: Patch voltage clamp electrophysiology allows for the direct measurement and quantification of block of voltage-gated sodium channels (NaV's), and allows the determination of the time- and voltage-dependence of block which has been interpreted as differential binding to the resting, open, and inactivated states of the sodium channel (Hille, B., Journal of General Physiology (1977), 69: 497-515).The following patch voltage clamp electrophysiology studies were performed on representative compounds of the invention using human embryonic kidney cells (HEK), permanently transfected with an expression vector containing the full-length cDNA coding for the desired human sodium channel alpha -subunit, grown in culture media containing 10% FBS, 1% PSG, and 0.5 mg/mL G418 at 37 C. with 5% CO2. HEK cells used for the electrophysiology (EP) recordings had a passage number of less than 40 for all studies and were used within three days from the time of plating.		210	ALA3638545	cell-based format	BindingDB Database
3.	ALA5264188	B	Displacement of [3H]GX-545 from human Nav1.7 alpha subunit expressed in HEK293 cells co-expressing beta1 subunit by liquid scintillation counting analysis	Homo sapiens	27	ALA5260838	cell-based format	Scientific Literature
4.	ALA5264189	B	Inhibition of human Nav1.7 alpha subunit expressed in HEK293 cells co-expressing beta1 subunit at -60 mV holding potential by whole-cell patch voltage clamp electrophysiology recording	Homo sapiens	27	ALA5260838	cell-based format	Scientific Literature
5.	ALA5264193	A	Inhibition of CYP3A4 (unknown origin)	Homo sapiens	3	ALA5260838	single protein format	Scientific Literature
6.	ALA5264194	A	Inhibition of CYP1A2 (unknown origin)	Homo sapiens	3	ALA5260838	single protein format	Scientific Literature
7.	ALA5264195	A	Inhibition of CYP2C19 (unknown origin)	Homo sapiens	3	ALA5260838	single protein format	Scientific Literature
8.	ALA5264196	A	Inhibition of CYP2C9 (unknown origin)	Homo sapiens	3	ALA5260838	single protein format	Scientific Literature
9.	ALA5264197	A	Inhibition of CYP2D6 (unknown origin)	Homo sapiens	3	ALA5260838	single protein format	Scientific Literature
10.	ALA5264201	A	Apparent permeability from apical to basolateral side in MDCK1 cells	Canis lupus familiaris	3	ALA5260838	cell-based format	Scientific Literature
11.	ALA5264202	A	Protein binding in human plasma	Homo sapiens	3	ALA5260838	cell-free format	Scientific Literature
12.	ALA5264203	A	Protein binding in mouse plasma	Mus musculus	3	ALA5260838	cell-free format	Scientific Literature
13.	ALA5264211	P	Lipophilicity, logD of the compound at pH 7.4		9	ALA5260838	small-molecule physicochemical format	Scientific Literature
14.	ALA5264212	B	Inhibition of mouse Nav1.7 at -60 mV holding potential by PatchXpress automated voltage clamp electrophysiology technique	Mus musculus	2	ALA5260838	single protein format	Scientific Literature
15.	ALA5264213	B	Inhibition of rat Nav1.7 at -60 mV holding potential by PatchXpress automated voltage clamp electrophysiology technique	Rattus norvegicus	2	ALA5260838	single protein format	Scientific Literature
16.	ALA5264214	B	Inhibition of human Nav1.5 alpha subunit expressed in HEK293 cells co-expressing beta1 subunit at -60 mV holding potential by PatchXpress automated voltage clamp electrophysiology technique	Homo sapiens	2	ALA5260838	cell-based format	Scientific Literature
17.	ALA5264215	B	Inhibition of human Nav1.2 alpha subunit expressed in HEK293 cells co-expressing beta1 subunit at -35 mV holding potential by PatchXpress automated voltage clamp electrophysiology technique	Homo sapiens	2	ALA5260838	cell-based format	Scientific Literature
18.	ALA5264216	B	Inhibition of human Nav1.1 alpha subunit expressed in HEK293 cells co-expressing beta1 subunit at -40 mV holding potential by PatchXpress automated voltage clamp electrophysiology technique	Homo sapiens	2	ALA5260838	cell-based format	Scientific Literature
19.	ALA5264217	B	Inhibition of human Nav1.6 alpha subunit expressed in HEK293 cells co-expressing beta1 subunit at -35 mV holding potential by PatchXpress automated voltage clamp electrophysiology technique	Homo sapiens	2	ALA5260838	cell-based format	Scientific Literature
20.	ALA5264218	B	Inhibition of TTX-resistant Nav1.8 in mouse dorsal root ganglion neuron at 10 uM by voltage clamp recording relative to control	Mus musculus	2	ALA5260838	tissue-based format	Scientific Literature