# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA712287 | B | In vitro inhibition of MGMT using cell free extracts from HeLa S3 cells | Homo sapiens | 12 | cell-based format | Scientific Literature | ||
2. | ALA752886 | B | AGT-inactivating activity was determined by 50% inactivation in HT-29 cell free extract upon incubation for 30 min | 22 | cell-based format | Scientific Literature | |||
3. | ALA752887 | B | AGT-inactivating activity was determined by 50% inactivation in HT-29 cells upon incubation for 4 h | 22 | cell-based format | Scientific Literature | |||
4. | ALA4262999 | B | Inhibition of human MPO assessed as reduction in enzyme-mediated chlorination by measuring reduction in HOCl formation at 10 uM preincubated for 10 mins followed by H2O2/NaCl addition and measured at 30 secs time interval for 5 mins by APF dye based fluorescence assay relative to control | Homo sapiens | 4 | single protein format | Scientific Literature | ||
5. | ALA4263000 | F | Hypochlorus scavenging activity at 10 uM in presence of sodium hypochlorite after 15 mins by APF dye based fluorescence assay relative to control | 4 | assay format | Scientific Literature | |||
6. | ALA4263001 | B | Inhibition of human MPO assessed as reduction in enzyme-mediated chlorination by measuring reduction in HOCl formation preincubated for 10 mins followed by H2O2/NaCl addition and measured at 30 secs time interval for 5 mins by APF dye based fluorescence assay | Homo sapiens | 23 | single protein format | Scientific Literature | ||
7. | ALA4263002 | B | Inhibition of human MPO assessed as reduction in enzyme-mediated chlorination by measuring reduction in HOCl formation in presence of H2O2/NaCl measured at 30 secs time interval for 5 mins by APF dye based fluorescence assay | Homo sapiens | 4 | single protein format | Scientific Literature | ||
8. | ALA4263003 | B | Inhibition of human MPO peroxidase activity in presence of H2O2 measured at 30 secs time interval for 5 mins by amplex red reagent based fluorescence assay | Homo sapiens | 4 | single protein format | Scientific Literature | ||
9. | ALA4263004 | P | Redox activity assessed as conversion of resazurin reagent to resorufin at 10 uM after 30 mins by fluorescence assay relative to control | 4 | small-molecule physicochemical format | Scientific Literature | |||
10. | ALA4263006 | B | Reversible inhibition of human MPO peroxidase activity at 50 to 250 nM preincubated for up to 30 mins followed by H2O2 addition and 2 to 10 times compound dilution measured at 30 secs time interval for 5 mins by amplex red reagent based fluorescence assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
11. | ALA4263008 | B | Inhibition of recombinant human C-terminal His6-tagged TPO (15 to 839 residues) expressed in baculovirus infected insect cells assessed as reduction in H2O2-catalyzed 3,5-iodo tyrosine formation using 3-iodotyrosine and potassium iodide preincubated for 10 mins followed by 3,5-iodo tyrosine/potassium iodide/H2O2 addition and measured after 15 mins | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
12. | ALA4263009 | B | Inhibition of recombinant human LPO isoform-1 (26 to 712 residues) expressed in baculovirus infected insect cells assessed as reduction in H2O2-catalyzed 3,5-iodo tyrosine formation using 3-iodotyrosine and potassium iodide preincubated for 10 mins followed by 3,5-iodo tyrosine/potassium iodide/H2O2 addition and measured after 15 mins | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
13. | ALA4263010 | B | Inhibition of eosinophil peroxidase (unknown origin)assessed as reduction in H2O2-catalyzed 3-bromo tyrosine formation using tyrosine and potassium bromide preincubated for 10 mins followed by tyrosine/potassium bromide/H2O2 addition and measured after 15 mins by RP-UPLC analysis | Homo sapiens | 2 | single protein format | Scientific Literature | ||
14. | ALA4263011 | B | Inhibition of mouse C-terminal His10 tagged MPO (Met16 to Thr718 residues) assessed as reduction in enzyme-mediated chlorination by measuring reduction in HOCl formation preincubated for 10 mins followed by H2O2/NaCl addition and measured at 30 secs time interval for 5 mins by APF dye based fluorescence assay | Mus musculus | 2 | single protein format | Scientific Literature | ||
15. | ALA4263012 | B | Inhibition of MPO in human neutrophils assessed as reduction in PMA-stimulated HOCl production incubated for 3 mins and measured for 25 mins by luminol reagent based luminescence assay | Homo sapiens | 3 | single protein format | Scientific Literature | ||
16. | ALA4263014 | B | Substrate activity at human MPO assessed as reversion of enzyme to resting state at 40 uM in presence of H2O2 by stopped flow assay | Homo sapiens | 2 | single protein format | Scientific Literature | ||
17. | ALA4263015 | B | Selectivity ratio of IC50 for recombinant human C-terminal His6-tagged TPO (15 to 839 residues) expressed in baculovirus infected insect cells to IC50 for human MPO | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
18. | ALA4263016 | B | Selectivity ratio of IC50 for recombinant human LPO isoform-1 (26 to 712 residues) expressed in baculovirus infected insect cells to IC50 for human MPO | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
19. | ALA4263017 | A | Inhibition of CYP3A4 (unknown origin) | Homo sapiens | 1 | single protein format | Scientific Literature | ||
20. | ALA4263020 | A | Protein binding in human plasma | Homo sapiens | 1 | cell-free format | Scientific Literature |