#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3705172	Binding	Radio Ligand Binding Assay: Serial dilutions of compounds (stock in 10 mM DMSO) are prepared by first diluting the compounds in DMSO followed by a 1:50 dilution into assay buffer (10 mM HEPES, pH 8.0, 100 mM NaCl, 5 mM MgCl2, 1 mM CaCl2, 0.5% fatty acid-free BSA, 0.05% Tween-20). The radioligand [3H]4 (example 39) (specific activity 1500 GBq/mmol) is diluted directly into the assay buffer immediately before use to obtain a 20 nM solution. The desired amount of membranes (20 μg/well) is diluted with assay buffer. 50 μL of pre-diluted compound and 50 μL of [3H]4 (example 39) is placed into the bottom of a 96-well well plate. 100 μL of the membrane-suspension is added and the plate stirred for 60 minutes. The reaction is stopped by transfer onto the filter of a 96-well GF/C filter plate (soaked for 1 hour in 0.25% PEI) using a cell harvester.	Homo sapiens	37	ALA3638598	assay format	BindingDB Database
2.	ALA4178741	Functional	Antagonist activity at human GPR4 expressed in human HeLa cells assessed as inhibition of IBMX-induced cAMP accumulation after 15 mins by HTRF assay	Homo sapiens	13	ALA4177561	cell-based format	Scientific Literature
3.	ALA4178742	Binding	Displacement of [3H]dofetilide from recombinant human ERG expressed in HEK293 cell membranes after 90 mins by liquid scintillation counting method	Homo sapiens	13	ALA4177561	cell-based format	Scientific Literature
4.	ALA4178743	Binding	Selectivity ratio of IC50 for displacement of [3H]dofetilide from recombinant human ERG expressed in HEK293 cell membranes to IC50 for human GPR4 expressed in HeLa cells	Homo sapiens	13	ALA4177561	cell-based format	Scientific Literature
5.	ALA4178744	Binding	Displacement of [3H]-R(-)-alpha-Methyl[imidazole-2.5(n)]histamine from human recombinant histamine H3 receptor expressed in CHOK1 cell membranes after 1 hr by scintillation counting method	Homo sapiens	13	ALA4177561	cell-based format	Scientific Literature
6.	ALA4178745	ADME	Clearance in Sprague-Dawley rat at 1 mg/kg, iv	Rattus norvegicus	11	ALA4177561	organism-based format	Scientific Literature
7.	ALA4178746	ADME	Dose normalized AUC in Sprague-Dawley rat at 3 mg/kg, po	Rattus norvegicus	11	ALA4177561	organism-based format	Scientific Literature