#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3705172	Binding	Radio Ligand Binding Assay: Serial dilutions of compounds (stock in 10 mM DMSO) are prepared by first diluting the compounds in DMSO followed by a 1:50 dilution into assay buffer (10 mM HEPES, pH 8.0, 100 mM NaCl, 5 mM MgCl2, 1 mM CaCl2, 0.5% fatty acid-free BSA, 0.05% Tween-20). The radioligand [3H]4 (example 39) (specific activity 1500 GBq/mmol) is diluted directly into the assay buffer immediately before use to obtain a 20 nM solution. The desired amount of membranes (20 μg/well) is diluted with assay buffer. 50 μL of pre-diluted compound and 50 μL of [3H]4 (example 39) is placed into the bottom of a 96-well well plate. 100 μL of the membrane-suspension is added and the plate stirred for 60 minutes. The reaction is stopped by transfer onto the filter of a 96-well GF/C filter plate (soaked for 1 hour in 0.25% PEI) using a cell harvester.	Homo sapiens	37	ALA3638598	assay format	BindingDB Database
2.	ALA4044420	Functional	Antagonist activity at human GPR4 expressed in human HeLa cells assessed as inhibition of pH-induced cAMP accumulation preincubated for 15 mins followed by cAMP addition measured after 60 mins by HTRF method	Homo sapiens	22	ALA4043105	cell-based format	Scientific Literature
3.	ALA4044421	Functional	Antagonist activity at mouse GPR4 expressed in HEK cells assessed as inhibition of pH-induced cAMP accumulation preincubated for 15 mins followed by cAMP addition measured after 60 mins by HTRF method	Mus musculus	11	ALA4043105	cell-based format	Scientific Literature
4.	ALA4044422	Functional	Antagonist activity at rat GPR4 expressed in HEK cells assessed as inhibition of pH-induced cAMP accumulation preincubated for 15 mins followed by cAMP addition measured after 60 mins by HTRF method	Rattus norvegicus	11	ALA4043105	cell-based format	Scientific Literature
5.	ALA4044425	ADME	Clearance in Sprague-Dawley rat at 1 mg/kg, iv via bolus administration by LC-MS/MS analysis	Rattus norvegicus	3	ALA4043105	organism-based format	Scientific Literature
6.	ALA4044426	ADME	Terminal half life in Sprague-Dawley rat at 1 mg/kg, iv via bolus administration by LC-MS/MS analysis	Rattus norvegicus	3	ALA4043105	organism-based format	Scientific Literature
7.	ALA4044427	ADME	Dose normalized AUC in Sprague-Dawley rat at 3 mg/kg, po administered via gavage by LC-MS/MS analysis	Rattus norvegicus	3	ALA4043105	organism-based format	Scientific Literature
8.	ALA4044428	ADME	Dose normalized Cmax in Sprague-Dawley rat at 1 mg/kg, iv via bolus administration or 3 mg/kg, po administered via gavage by LC-MS/MS analysis	Rattus norvegicus	3	ALA4043105	organism-based format	Scientific Literature
9.	ALA4044429	ADME	Oral bioavailability in Sprague-Dawley rat at 3 mg/kg administered via gavage by LC-MS/MS analysis	Rattus norvegicus	3	ALA4043105	organism-based format	Scientific Literature
10.	ALA4044430	Functional	In vivo antiangiogenic activity in po bid dosed FVB mouse assessed as inhibition of VEGF-induced angiogenesis administered for 3 days starting from 5 hrs prior to implantation measured on day 4 post implantation	Mus musculus	1	ALA4043105	organism-based format	Scientific Literature
11.	ALA4044431	Functional	Anti-inflammatory activity in po dosed Lewis rat antigen-induced arthritis model assessed as inhibition of knee swelling administered bid via gavage measured after 7 days	Rattus norvegicus	1	ALA4043105	organism-based format	Scientific Literature
12.	ALA4044432	Functional	Anti-inflammatory activity in Lewis rat antigen-induced arthritis model assessed as inhibition of joint damage or erosion at 90 mg/kg, po bid administered via gavage measured after 7 days	Rattus norvegicus	1	ALA4043105	organism-based format	Scientific Literature
13.	ALA4044433	Functional	Antinociceptive activity in CFA-induced Wistar Han rat hyperalgesia model assessed as increase in paw withdrawal threshold at 10 to 30 mg/kg, po administered via gavage on day3 post CFA challenge measured up to 6 hrs post dose	Rattus norvegicus	1	ALA4043105	organism-based format	Scientific Literature
14.	ALA4044434	Binding	Antagonist activity at histamine H3 receptor (unknown origin)	Homo sapiens	1	ALA4043105	single protein format	Scientific Literature
15.	ALA4044435	Binding	Displacement of [3H]-dofetilide from recombinant human ERG expressed in HEK293 cells	Homo sapiens	1	ALA4043105	cell-based format	Scientific Literature
16.	ALA4044436	Toxicity	Toxicity in Wistar Han rat CFA-induced hyperalgesia model	Rattus norvegicus	1	ALA4043105	assay format	Scientific Literature
17.	ALA4044437	Toxicity	Toxicity in Lewis rat mBSA-induced arthritis model	Rattus norvegicus	1	ALA4043105	assay format	Scientific Literature
18.	ALA4044438	Toxicity	Toxicity in FVB mouse VEGF-induced angiogenesis model	Mus musculus	1	ALA4043105	assay format	Scientific Literature
19.	ALA4044440	Functional	Anti-inflammatory activity in Lewis rat mBSA-induced arthritis model assessed as inhibition of mBSA-induced knee swelling at 10 mg/kg, po, administered bid via gavage measured after 7 days post last dose relative to control	Rattus norvegicus	1	ALA4043105	organism-based format	Scientific Literature
20.	ALA4044441	Functional	Anti-inflammatory activity in Lewis rat mBSA-induced arthritis model assessed as inhibition of mBSA-induced knee swelling at 30 mg/kg, po, bid administered bid via gavage measured after 7 days post last dose relative to control	Rattus norvegicus	1	ALA4043105	organism-based format	Scientific Literature