#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3706186	B	In Vitro HPLC Assay: This assay was used to determine the inhibiting effect of the inhibitors in a concentration of 1 mM. A standard incubation mixture consisted of 75 μl of 50 mM potassium phosphate buffer pH 7.4 and contained NMMA in a concentration of 200 μM. The inhibitors were used in final concentrations of 1 mM. By adding 2 μg of hDDAH-1, the reaction was started and the samples were incubated for 30 minutes at 37° C. in, a shaking water bath. The reaction was then stopped by adding 75 μl of acetonitrile, the samples were shaken for 5′ minutes and centrifuged (12,000 g, 5 min). The supernatant was added to the HPLC.	Homo sapiens	2		single protein format	BindingDB Database
2.	ALA4380142	B	Inhibition of 6xHis-tagged recombinant human DDAH1 expressed in Escherichia coli BL21 cells using NMMA as substrate measured after 30 mins by colder reagent based microplate reader assay	Homo sapiens	18		cell-based format	Scientific Literature
3.	ALA4380143	B	Inhibition of bovine liver arginase at 1 mM using L-arginine as substrate measured after 30 mins by colder reagent based microplate reader assay relative to control	Bos taurus	16		assay format	Scientific Literature
4.	ALA4380144	B	Inhibition of neuronal NOS (unknown origin) assessed as formation of methemoglobin from oxyhemoglobin at 1 mM using L-arginine as substrate in presence of oxyhemoglobin and NADPH by UV-vis spectrophotometry relative to control	Homo sapiens	16		single protein format	Scientific Literature
5.	ALA4380145	B	Inhibition of endothelial NOS (unknown origin) assessed as formation of methemoglobin from oxyhemoglobin at 1 mM using L-arginine as substrate in presence of oxyhemoglobin and NADPH by UV-vis spectrophotometry relative to control	Homo sapiens	16		single protein format	Scientific Literature
6.	ALA4380146	B	Inhibition of iNOS (unknown origin) assessed as formation of methemoglobin from oxyhemoglobin at 1 mM using L-arginine as substrate in presence of oxyhemoglobin and NADPH by UV-vis spectrophotometry relative to control	Homo sapiens	16		single protein format	Scientific Literature
7.	ALA4380153	A	Cytotoxicity against human HEK293T cells assessed as effect on cell number at 0.1 to 1000 uM incubated for overnight by DAPI staining based analysis	Homo sapiens	2		cell-based format	Scientific Literature
8.	ALA4380154	A	Cytotoxicity against human HEK293T cells assessed as effect on cell viability by measuring ATP content at 0.1 to 1000 uM incubated for overnight by CellTiter-Glo luminescent analysis	Homo sapiens	2		cell-based format	Scientific Literature