#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3705899	B	In Vitro Assay: Recombinant human factor D (expressed in E. coli and purified using standard methods) at 10 nM concentration is incubated with test compound at various concentrations for 1 hour at room temperature in 0.1 M Hepes buffer, pH 7.5, containing 1 mM MgCl2, 1 M NaCl and 0.05% CHAPS. A synthetic substrate Z-Lys-thiobenzyl and 2,4-dinitrobenzenesulfonyl-fluoresceine are added to final concentrations of 200 μM and 25 μM, respectively. The increase in fluorescence is recorded at excitation of 485 nm and emission at 535 nm in a microplate spectrofluorimeter. IC50 values are calculated from percentage of inhibition of complement factor D-activity as a function of test compound concentration.		768		assay format	BindingDB Database
2.	ALA4013288	B	Inhibition of recombinant human complement factor D catalytic domain using Z-Lys-thiobenzylester as substrate preincubated for 1 hr followed by substrate addition by 2,4-dinitrobenzenesulfonyl-2,7-desmethyl-fluorecein probe based spectrofluorimetric assay	Homo sapiens	25		single protein format	Scientific Literature
3.	ALA4013310	A	Protein binding in human plasma	Homo sapiens	14		cell-free format	Scientific Literature
4.	ALA4013311	B	Inhibition of complement factor D in human whole blood assessed as decrease in zymosan-induced AP activation mediated soluble MAC complex formation preincubated for 15 mins followed by zymosan addition measured after 40 mins by ELISA	Homo sapiens	14		single protein format	Scientific Literature
5.	ALA4013312	A	Inhibition of CYP2C9 (unknown origin) using diclofenac as substrate	Homo sapiens	10		single protein format	Scientific Literature
6.	ALA4013313	A	Apparent permeability from apical to basolateral side in human Caco2 cells	Homo sapiens	10		cell-based format	Scientific Literature
7.	ALA4013314	A	Apparent permeability from basolateral to apical side in human Caco2 cells	Homo sapiens	10		cell-based format	Scientific Literature
8.	ALA4013315	A	Efflux ratio of apparent permeability across basolateral to apical side over apical to basolateral side in human Caco2 cells	Homo sapiens	10		cell-based format	Scientific Literature
9.	ALA4013316	P	Aqueous equilibrium solubility of compound in pH 6.8 solution by high throughput assay		10		small-molecule physicochemical format	Scientific Literature