#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3705225	B	Homogeneous Time-Resolved Fluorescence (HTRF) Assay : A recombinant GST-hSYK fusion protein was used to measure potency of compounds to inhibit human SYK activity. The recombinant human GST-SYK (Carna Biosciences #08-176) (5 pM final concentration) was incubated with various concentrations of the inhibitor diluted in DMSO (0.1% final concentration) for 10 minutes at room temperature in 15 mM Tris-HCl (pH 7.5), 0.01% tween 20, 2 mM DTT in 384 well plate format. To initiate the reaction the biotinylated substrate peptide (250 nM final concentration) that contains the phosphorylation site for SYK was added with magnesium (5 mM final concentration) and ATP (25 μM final concentration). Final volume of the reaction was 10 μL. Phosphorylation of the peptide was allowed to proceed for 45′ at room temperature.	Homo sapiens	46		single protein format	BindingDB Database
2.	ALA4725212	T	Incucyte cell viability with HEK293T	Homo sapiens	1,713		cell-based format	EUbOPEN Chemogenomic Library
3.	ALA4725213	T	Incucyte cell viability with human fibroblast	Homo sapiens	1,718		cell-based format	EUbOPEN Chemogenomic Library
4.	ALA4725214	T	Incucyte cell viability with U2OS	Homo sapiens	1,712		cell-based format	EUbOPEN Chemogenomic Library
5.	ALA4879555	B	KinomeScan assay: inhibition of AAK1	Homo sapiens	108		single protein format	Donated Chemical Probes - SGC Frankfurt
6.	ALA4879556	B	KinomeScan assay: inhibition of ABL1(E255K)-phosphorylated	Homo sapiens	108		single protein format	Donated Chemical Probes - SGC Frankfurt
7.	ALA4879557	B	KinomeScan assay: inhibition of ABL1(F317I)-nonphosphorylated	Homo sapiens	108		single protein format	Donated Chemical Probes - SGC Frankfurt
8.	ALA4879558	B	KinomeScan assay: inhibition of ABL1(F317I)-phosphorylated	Homo sapiens	108		single protein format	Donated Chemical Probes - SGC Frankfurt
9.	ALA4879559	B	KinomeScan assay: inhibition of ABL1(F317L)-nonphosphorylated	Homo sapiens	108		single protein format	Donated Chemical Probes - SGC Frankfurt
10.	ALA4879560	B	KinomeScan assay: inhibition of ABL1(F317L)-phosphorylated	Homo sapiens	108		single protein format	Donated Chemical Probes - SGC Frankfurt
11.	ALA4879561	B	KinomeScan assay: inhibition of ABL1(H396P)-nonphosphorylated	Homo sapiens	108		single protein format	Donated Chemical Probes - SGC Frankfurt
12.	ALA4879562	B	KinomeScan assay: inhibition of ABL1(H396P)-phosphorylated	Homo sapiens	108		single protein format	Donated Chemical Probes - SGC Frankfurt
13.	ALA4879563	B	KinomeScan assay: inhibition of ABL1(M351T)-phosphorylated	Homo sapiens	108		single protein format	Donated Chemical Probes - SGC Frankfurt
14.	ALA4879564	B	KinomeScan assay: inhibition of ABL1(Q252H)-nonphosphorylated	Homo sapiens	108		single protein format	Donated Chemical Probes - SGC Frankfurt
15.	ALA4879565	B	KinomeScan assay: inhibition of ABL1(Q252H)-phosphorylated	Homo sapiens	108		single protein format	Donated Chemical Probes - SGC Frankfurt
16.	ALA4879566	B	KinomeScan assay: inhibition of ABL1(T315I)-nonphosphorylated	Homo sapiens	108		single protein format	Donated Chemical Probes - SGC Frankfurt
17.	ALA4879567	B	KinomeScan assay: inhibition of ABL1(T315I)-phosphorylated	Homo sapiens	108		single protein format	Donated Chemical Probes - SGC Frankfurt
18.	ALA4879568	B	KinomeScan assay: inhibition of ABL1(Y253F)-phosphorylated	Homo sapiens	108		single protein format	Donated Chemical Probes - SGC Frankfurt
19.	ALA4879569	B	KinomeScan assay: inhibition of ABL1-nonphosphorylated	Homo sapiens	108		single protein format	Donated Chemical Probes - SGC Frankfurt
20.	ALA4879570	B	KinomeScan assay: inhibition of ABL1-phosphorylated	Homo sapiens	108		single protein format	Donated Chemical Probes - SGC Frankfurt