#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3706045	Binding	Inhibition Assay: The ability of the nuclear factor-kappa B (NF-kB)-inducing kinase (NIK) to catalyze the hydrolysis of adenosine-5'-triphosphate (ATP) was monitored using the Transcreener ADP (adenosine-5'-diphosphate) assay (BellBrook Labs). Purified NIK (0.2-1 nM) derived from a baculovirus-infected insect cell expression system was incubated with test compounds for 1-3.5 hours in 50 mM 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid buffer (pH 7.2) containing 10 mM MgCl.sub.2, 2 mM dithiothreitol, 10 uM ATP, 0.01% Triton X-100, 0.1% gamma-globulins from bovine blood, 1% dimethylsulfoxide (DMSO), 12 ug/mL ADP antibody and 4 nM ADP-AlexaFluor.RTM. 633 tracer. Reactions were quenched by the addition of 20 mM 2,2',2'',2'''-(ethane-1,2-diyldinitrilo)tetraacetic acid and 0.01% Brij 35. The tracer bound to the antibody was displaced by the ADP generated during the NIK reaction, which causes a decrease in fluorescence polarization that was measured by laser excitation.		608	ALA3638554	assay format	BindingDB Database
2.	ALA4052941	Binding	Inhibition of TNF-alpha-stimulated RELA translocation from cytoplasm to nucleus in human HeLa cells preincubated for 4.5 hrs followed by TNF-alpha stimulation measured after 30 mins	Homo sapiens	3	ALA4052585	cell-based format	Scientific Literature
3.	ALA4052942	Binding	Inhibition of NIK (unknown origin) after 1 to 2 hrs by ADP-FP assay	Homo sapiens	30	ALA4052585	single protein format	Scientific Literature
4.	ALA4052943	Physicochemical	Lipophilicity, log D of the compound at pH 7.4 by shake flask method		24	ALA4052585	small-molecule physicochemical format	Scientific Literature
5.	ALA4052944	Binding	Inhibition of NIK (unknown origin) expressed in HEK293 cells assessed as decrease in NFkappaB signal after 24 hrs by dual glo luciferase reporter gene assay	Homo sapiens	3	ALA4052585	cell-based format	Scientific Literature
6.	ALA4052945	ADME	Cytotoxicity against HEK293 cells assessed as effect on cell viability after 24 hrs by HSV-TK reporter assay	Homo sapiens	3	ALA4052585	cell-based format	Scientific Literature
7.	ALA4052946	Binding	Inhibition of NIK in human HeLa cells assessed as decrease in antilymphotoxin-beta receptor antibody-stimulated p52 translocation from cytoplasm to nucleus after 5 hrs	Homo sapiens	3	ALA4052585	cell-based format	Scientific Literature
8.	ALA4052947	Binding	Inhibition of N-terminal His6-tagged recombinant full-length human p110delta/untagged recombinant full length human p85alpha expressed in baculovirus infected Sf21 insect cells using PIP2:3PS lipid substrate after 120 mins by ADP-Glo assay	Homo sapiens	19	ALA4052585	cell-based format	Scientific Literature
9.	ALA4052951	Binding	Inhibition of recombinant GST-tagged human NIK expressed in baculovirus expression system at 0.1 uM relative to control	Homo sapiens	2	ALA4052585	assay format	Scientific Literature
10.	ALA4052952	ADME	Apparent permeability across MDCK cells at 100 uM	Canis lupus familiaris	2	ALA4052585	cell-based format	Scientific Literature
11.	ALA4052953	Physicochemical	Thermodynamic solubility of compound at pH 7.4		2	ALA4052585	small-molecule physicochemical format	Scientific Literature
12.	ALA4052954	ADME	Clearance in human liver microsomes	Homo sapiens	2	ALA4052585	assay format	Scientific Literature
13.	ALA4052955	ADME	Clearance in rat liver microsomes	Rattus norvegicus	2	ALA4052585	assay format	Scientific Literature
14.	ALA4052956	ADME	Clearance in mouse liver microsomes	Mus musculus	2	ALA4052585	assay format	Scientific Literature
15.	ALA4052957	ADME	Clearance in dog liver microsomes	Canis lupus familiaris	2	ALA4052585	assay format	Scientific Literature
16.	ALA4052958	ADME	Clearance in cynomolgus monkey liver microsomes	Macaca fascicularis	2	ALA4052585	assay format	Scientific Literature
17.	ALA4052959	ADME	Protein binding in human plasma	Homo sapiens	2	ALA4052585	cell-free format	Scientific Literature
18.	ALA4052960	ADME	Plasma clearance in Sprague-Dawley rat	Rattus norvegicus	2	ALA4052585	assay format	Scientific Literature
19.	ALA4052961	ADME	Volume of distribution in Sprague-Dawley rat	Rattus norvegicus	2	ALA4052585	assay format	Scientific Literature
20.	ALA4052962	ADME	Oral bioavailability in Sprague-Dawley rat at 5 mg/kg	Rattus norvegicus	2	ALA4052585	organism-based format	Scientific Literature