| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3706032 | B | Binding Assay: The binding affinity of the MDM2 inhibitors disclosed herein was determined using a fluorescence polarization-based (FP-based) binding assay using a recombinant human His-tagged MDM2 protein (residues 1-118) and a fluorescently tagged p53-based peptide. The design of the fluorescence probe was based upon a previously reported high-affinity p53-based peptidomimetic compound called PMDM6-F (García-Echeverria et al., J. Med. Chem. 43: 3205-3208 (2000)). | Homo sapiens | 39 | single protein format | BindingDB Database | ||
| 2. | ALA4011380 | B | Inhibition of FAM-tagged p53-based PMDM6-F peptide binding to human recombinant His-tagged MDM2 (1 to 118 residues) after 30 mins by fluorescence polarization assay | Homo sapiens | 52 | single protein format | Scientific Literature | ||
| 3. | ALA4323192 | B | Displacement of PMDM6-F fluorescent probe from human recombinant His-tagged MDM2 (1 to 118 residues) after 15 mins by fluorescence polarization assay | Homo sapiens | 6 | single protein format | Scientific Literature | ||
| 4. | ALA4323194 | F | Antiproliferative activity against human RS4:11 cells expressing wild type p53 assessed as cell growth inhibition after 4 days by WST-8 assay | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
| 5. | ALA4323195 | F | Antiproliferative activity against human MOLM13 cells expressing wild type p53 assessed as cell growth inhibition after 4 days by WST-8 assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 6. | ALA4323196 | F | Antiproliferative activity against human MV4-11 cells expressing wild type p53 assessed as cell growth inhibition after 4 days by WST-8 assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 7. | ALA4323197 | F | Antiproliferative activity against human HL60 cells with lack of p53 assessed as cell growth inhibition after 4 days by WST-8 assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 8. | ALA4323198 | F | Antiproliferative activity against human RS4:11/IRMI-2 cells harboring p53 hotspot mutation Y236H/R249G assessed as cell growth inhibition after 4 days by WST-8 assay | Homo sapiens | 9 | cell-based format | Scientific Literature | ||
| 9. | ALA4323199 | F | Antiproliferative activity against human MDA-MB-231 cells harboring mutant p53 assessed as cell growth inhibition after 4 days by WST-8 assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 10. | ALA4323200 | F | Antiproliferative activity against human MDA-MB-468 cells harboring mutant p53 assessed as cell growth inhibition after 4 days by WST-8 assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 11. | ALA4323202 | B | Induction of MDM2 degradation in human RS4:11 cells assessed as upregulation of MDM2 at 1000 to 3000 nM after 1 hr by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 12. | ALA4323203 | B | Induction of MDM2 degradation in human RS4:11 cells assessed as upregulation of p53 at 1000 to 3000 nM after 1 hr by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 13. | ALA4323216 | F | Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition after 4 days in presence of control siRNA for 2 days followed by compound treatment by WST-8 assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 14. | ALA4323217 | F | Antiproliferative activity against human MDA-MB-468 cells assessed as cell growth inhibition after 4 days in presence of control siRNA for 2 days followed by compound treatment by WST-8 assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 15. | ALA4323218 | F | Antiproliferative activity against human MDA-MB-231 cells transfected with MDM2 siRNA assessed as cell growth inhibition after 4 days by WST-8 assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 16. | ALA4323219 | F | Antiproliferative activity against human MDA-MB-468 cells transfected with MDM2 siRNA assessed as cell growth inhibition after 4 days by WST-8 assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 17. | ALA4323220 | B | Binding affinity to cereblon in human RV4:11 cells expressing wild type p53 assessed as cell growth inhibition measured after 4 days | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 18. | ALA4323221 | B | Binding affinity to cereblon in human RV4:11/IRMI-2 cells harboring p53 hotspot mutation Y236H/R249G assessed as cell growth inhibition measured after 4 days | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 19. | ALA4323222 | B | Binding affinity to cereblon in human MDA-MB-231 cells assessed as cell growth inhibition measured after 4 days | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 20. | ALA4323223 | B | Binding affinity to cereblon in human MDA-MB-468 cells assessed as cell growth inhibition measured after 4 days | Homo sapiens | 3 | cell-based format | Scientific Literature |
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