#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3705774	B	Electrophysiology Assay: Block of Kir 1.1 (ROMKl) currents was examined by whole cell voltage clamp (Hamill et. al. Pfluegers Archives 391 : 85- 100 (1981)) using the IonWorks Quattro automated electrophysiology platform (Molecular Devices, Sunnyvale, CA). Chinese hamster ovary cells stably expressing Kirl.l channels were maintained in T-75 flasks in cell culture media in a humidified 10% CO2 incubator at 37 0C. Prior to an experiment, Kirl .l expression was induced by overnight incubation with 1 mM sodium butyrate. On the day of the experiment, cells were dissociated with 2.5 ml of Versene (Invitrogen 15040-066) for approximately 6 min at 37 0C and suspended in 10 ml of bath solution containing (in roM): 150 NaCl, 10 KCl, 2.7 CaCl2, 0.5 MgCl2, 5 HEPES5 pH 7.4. After centrifugation, the cell pellet was resuspended in approximately 4.0 ml of bath solution and placed in the Ion Works instrument. The intracellular solution consisted of (in mM): 80 K gluconate, 40 KCl, 20 KF5 3.2 MgCl2, 3 EGTA, 5 Hepes, pH 7.4.		9	ALA3638489	cell-based format	BindingDB Database
2.	ALA4427467	B	Inhibition of ROMK (unknown origin) after 30 mins by 86Rb+ efflux assay	Homo sapiens	13	ALA4425169	single protein format	Scientific Literature
3.	ALA4427468	B	Displacement of [35S]MK-499 from human ERG	Homo sapiens	13	ALA4425169	single protein format	Scientific Literature
4.	ALA4427470	B	Inhibition of ROMK (unknown origin) expressed in CHO cells after 6 mins by electrophysiological assay	Homo sapiens	5	ALA4425169	cell-based format	Scientific Literature
5.	ALA4427471	B	Inhibition of human ERG after 6 mins by electrophysiological assay	Homo sapiens	5	ALA4425169	single protein format	Scientific Literature
6.	ALA4427472	B	Selectivity ratio of IC50 for human ERG to IC50 for ROMK (unknown origin) expressed in CHO cells by electrophysiological assay	Homo sapiens	5	ALA4425169	cell-based format	Scientific Literature
7.	ALA4427473	B	Inhibition of ROMK (unknown origin) after 30 mins by Tl+ influx assay	Homo sapiens	3	ALA4425169	single protein format	Scientific Literature
8.	ALA4427474	F	Diuretic activity in Sprague-Dawley rat at 3 mg/kg via oral gavage administered 30 mins prior to void induction by loading saline measured upto 4 hrs relative to vehicle treated control	Rattus norvegicus	5	ALA4425169	organism-based format	Scientific Literature
9.	ALA4427476	F	Natriuretic activity in Sprague-Dawley rat at 3 mg/kg via oral gavage administered 30 mins prior to void induction by loading saline measured upto 4 hrs relative to vehicle treated control	Rattus norvegicus	5	ALA4425169	organism-based format	Scientific Literature
10.	ALA4427478	F	Diuretic activity in Sprague-Dawley rat at 0.3 mg/kg via oral gavage administered 30 mins prior to void induction by loading saline measured upto 4 hrs relative to vehicle treated control	Rattus norvegicus	2	ALA4425169	organism-based format	Scientific Literature
11.	ALA4427479	F	Diuretic activity in Sprague-Dawley rat at 1 mg/kg via oral gavage administered 30 mins prior to void induction by loading saline measured upto 4 hrs relative to vehicle treated control	Rattus norvegicus	3	ALA4425169	organism-based format	Scientific Literature
12.	ALA4427480	F	Natriuretic activity in Sprague-Dawley rat at 0.3 mg/kg via oral gavage administered 30 mins prior to void induction by loading saline measured upto 4 hrs relative to vehicle treated control	Rattus norvegicus	2	ALA4425169	organism-based format	Scientific Literature
13.	ALA4427481	F	Natriuretic activity in Sprague-Dawley rat at 1 mg/kg via oral gavage administered 30 mins prior to void induction by loading saline measured upto 4 hrs relative to vehicle treated control	Rattus norvegicus	3	ALA4425169	organism-based format	Scientific Literature
14.	ALA4427482	F	Kaliuretic activity in Sprague-Dawley rat at 3 mg/kg via oral gavage administered 30 mins prior to void induction by loading saline measured upto 4 hrs relative to vehicle treated control	Rattus norvegicus	3	ALA4425169	organism-based format	Scientific Literature
15.	ALA4427484	F	Kaliuretic activity in Sprague-Dawley rat at 0.3 mg/kg via oral gavage administered 30 mins prior to void induction by loading saline measured upto 4 hrs relative to vehicle treated control	Rattus norvegicus	2	ALA4425169	organism-based format	Scientific Literature
16.	ALA4427485	F	Kaliuretic activity in Sprague-Dawley rat at 1 mg/kg via oral gavage administered 30 mins prior to void induction by loading saline measured upto 4 hrs relative to vehicle treated control	Rattus norvegicus	3	ALA4425169	organism-based format	Scientific Literature
17.	ALA4427491	A	Clearance in rat at 0.5 mg/kg, iv or 1 mg/kg, po	Rattus norvegicus	2	ALA4425169	organism-based format	Scientific Literature
18.	ALA4427492	A	Volume of distribution at steady state in rat at 0.5 mg/kg, iv or 1 mg/kg, po	Rattus norvegicus	2	ALA4425169	organism-based format	Scientific Literature
19.	ALA4427493	A	Dose normalized AUC in rat at 1 mg/kg, po	Rattus norvegicus	2	ALA4425169	organism-based format	Scientific Literature
20.	ALA4427494	A	Half life in rat at 0.5 mg/kg, iv or 1 mg/kg, po	Rattus norvegicus	2	ALA4425169	organism-based format	Scientific Literature